BiP modulates the affinity of its co-chaperone ERj1 for ribosomes

Julia Benedix, Patrick Lajoie, Himjyot Jaiswal, Carsten Burgard, Markus Greiner, Richard Zimmermann, Sabine Rospert, Erik L. Snapp, Johanna Dudek

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Ribosomes synthesizing secretory and membrane proteins are bound to the endoplasmic reticulum (ER) membrane and attach to ribosome-associated membrane proteins such as the Sec61 complex, which forms the protein-conducting channel in the membrane. The ER membrane-resident Hsp40 protein ERj1 was characterized as being able to recruit BiP to ribosomes in solution and to regulate protein synthesis in a BiP-dependent manner. Here, we show that ERj1 and Sec61 are associated with ribosomes at the ER of human cells and that the binding of ERj1 to ribosomes occurs with a binding constant in the picomolar range and is prevented by pretreatment of ribosomes with RNase. However, the affinity of ERj1 for ribosomes dramatically changes upon binding of BiP. This modulation by BiP may be responsible for the dual role of ERj1 at the ribosome, i.e. acting as a recruiting factor for BiP and regulating translation.

Original languageEnglish (US)
Pages (from-to)36427-36433
Number of pages7
JournalJournal of Biological Chemistry
Volume285
Issue number47
DOIs
StatePublished - Nov 19 2010

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Ribosomes
Membranes
Membrane Proteins
Proteins
Endoplasmic Reticulum
Ribonucleases
Cells
Modulation
Ion Channels

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Benedix, J., Lajoie, P., Jaiswal, H., Burgard, C., Greiner, M., Zimmermann, R., ... Dudek, J. (2010). BiP modulates the affinity of its co-chaperone ERj1 for ribosomes. Journal of Biological Chemistry, 285(47), 36427-36433. https://doi.org/10.1074/jbc.M110.143263

BiP modulates the affinity of its co-chaperone ERj1 for ribosomes. / Benedix, Julia; Lajoie, Patrick; Jaiswal, Himjyot; Burgard, Carsten; Greiner, Markus; Zimmermann, Richard; Rospert, Sabine; Snapp, Erik L.; Dudek, Johanna.

In: Journal of Biological Chemistry, Vol. 285, No. 47, 19.11.2010, p. 36427-36433.

Research output: Contribution to journalArticle

Benedix, J, Lajoie, P, Jaiswal, H, Burgard, C, Greiner, M, Zimmermann, R, Rospert, S, Snapp, EL & Dudek, J 2010, 'BiP modulates the affinity of its co-chaperone ERj1 for ribosomes', Journal of Biological Chemistry, vol. 285, no. 47, pp. 36427-36433. https://doi.org/10.1074/jbc.M110.143263
Benedix J, Lajoie P, Jaiswal H, Burgard C, Greiner M, Zimmermann R et al. BiP modulates the affinity of its co-chaperone ERj1 for ribosomes. Journal of Biological Chemistry. 2010 Nov 19;285(47):36427-36433. https://doi.org/10.1074/jbc.M110.143263
Benedix, Julia ; Lajoie, Patrick ; Jaiswal, Himjyot ; Burgard, Carsten ; Greiner, Markus ; Zimmermann, Richard ; Rospert, Sabine ; Snapp, Erik L. ; Dudek, Johanna. / BiP modulates the affinity of its co-chaperone ERj1 for ribosomes. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 47. pp. 36427-36433.
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