A simple methodology for single-site conjugation of macrocycles and metallomacrocycles with biotin is presented. This method has been used to conjugate a redox-active macrocyclic complex and embed it into avidin. The resulting biotin-metal complex-avidin adduct exhibits peroxidase activity as shown by the oxidation of ABTS and pyrogallol with H2O2.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Inclusion Phenomena and Macrocyclic Chemistry|
|State||Published - Jun 2009|
ASJC Scopus subject areas
- Food Science
- Condensed Matter Physics