Biotinylation of aminopyridine-based macrocycles and metallomacrocycles and inclusion of biotinylated iron(II) complex in avidin

Voltaire G. Organo; Wanhua Ye; Prakhar K. Agarwal; Elena V. Rybak-Akimova

doi:10.1007/s10847-009-9531-z

Biotinylation of aminopyridine-based macrocycles and metallomacrocycles and inclusion of biotinylated iron(II) complex in avidin

Voltaire G. Organo, Wanhua Ye, Prakhar K. Agarwal, Elena V. Rybak-Akimova

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A simple methodology for single-site conjugation of macrocycles and metallomacrocycles with biotin is presented. This method has been used to conjugate a redox-active macrocyclic complex and embed it into avidin. The resulting biotin-metal complex-avidin adduct exhibits peroxidase activity as shown by the oxidation of ABTS and pyrogallol with H₂O₂.

Original language	English (US)
Pages (from-to)	15-21
Number of pages	7
Journal	Journal of Inclusion Phenomena and Macrocyclic Chemistry
Volume	64
Issue number	1-2
DOIs	https://doi.org/10.1007/s10847-009-9531-z
State	Published - Jun 2009
Externally published	Yes

Keywords

Biotinylation
Catalysis
Macrocycles
Metallomacrocycles

ASJC Scopus subject areas

Food Science
General Chemistry
Condensed Matter Physics

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10.1007/s10847-009-9531-z

Cite this

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title = "Biotinylation of aminopyridine-based macrocycles and metallomacrocycles and inclusion of biotinylated iron(II) complex in avidin",

abstract = "A simple methodology for single-site conjugation of macrocycles and metallomacrocycles with biotin is presented. This method has been used to conjugate a redox-active macrocyclic complex and embed it into avidin. The resulting biotin-metal complex-avidin adduct exhibits peroxidase activity as shown by the oxidation of ABTS and pyrogallol with H2O2.",

keywords = "Biotinylation, Catalysis, Macrocycles, Metallomacrocycles",

author = "Organo, {Voltaire G.} and Wanhua Ye and Agarwal, {Prakhar K.} and Rybak-Akimova, {Elena V.}",

note = "Funding Information: Acknowledgments This work was supported by the US Department of Energy (Grant DE-FG02-06ER15799). The NMR facility, the ESI-MS spectrometer, and rapid kinetic instrumentation at Tufts were supported by the NSF (grants CHE-9723772, MRI CHE 0821508, MRI CHE 0320783, and CHE 0639138).",

year = "2009",

month = jun,

doi = "10.1007/s10847-009-9531-z",

language = "English (US)",

volume = "64",

pages = "15--21",

journal = "Journal of Inclusion Phenomena and Macrocyclic Chemistry",

issn = "0923-0750",

publisher = "Kluwer Academic Publishers",

number = "1-2",

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TY - JOUR

T1 - Biotinylation of aminopyridine-based macrocycles and metallomacrocycles and inclusion of biotinylated iron(II) complex in avidin

AU - Organo, Voltaire G.

AU - Ye, Wanhua

AU - Agarwal, Prakhar K.

AU - Rybak-Akimova, Elena V.

N1 - Funding Information: Acknowledgments This work was supported by the US Department of Energy (Grant DE-FG02-06ER15799). The NMR facility, the ESI-MS spectrometer, and rapid kinetic instrumentation at Tufts were supported by the NSF (grants CHE-9723772, MRI CHE 0821508, MRI CHE 0320783, and CHE 0639138).

PY - 2009/6

Y1 - 2009/6

N2 - A simple methodology for single-site conjugation of macrocycles and metallomacrocycles with biotin is presented. This method has been used to conjugate a redox-active macrocyclic complex and embed it into avidin. The resulting biotin-metal complex-avidin adduct exhibits peroxidase activity as shown by the oxidation of ABTS and pyrogallol with H2O2.

AB - A simple methodology for single-site conjugation of macrocycles and metallomacrocycles with biotin is presented. This method has been used to conjugate a redox-active macrocyclic complex and embed it into avidin. The resulting biotin-metal complex-avidin adduct exhibits peroxidase activity as shown by the oxidation of ABTS and pyrogallol with H2O2.

KW - Biotinylation

KW - Catalysis

KW - Macrocycles

KW - Metallomacrocycles

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DO - 10.1007/s10847-009-9531-z

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JO - Journal of Inclusion Phenomena and Macrocyclic Chemistry

JF - Journal of Inclusion Phenomena and Macrocyclic Chemistry

IS - 1-2

ER -

Biotinylation of aminopyridine-based macrocycles and metallomacrocycles and inclusion of biotinylated iron(II) complex in avidin

Abstract

Keywords

ASJC Scopus subject areas

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