Biosynthesis of heterogeneous forms of multidrug resistance-associated glycoproteins

L. M. Greenberger, S. S. Williams, Susan Band Horwitz

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Multidrug-resistant J774.2 mouse macrophage-like cells, selected for resistance to colchicine, vinblastine, or taxol, overexpress antigenically related glycoproteins with distinct electrophoretic mobilities. These plasma membrane glycoproteins are likely to play a pivotal role in the expression of the multidrug resistance phenotype. To determine how these multidrug resistance-associated glycoproteins differ, the biosynthesis and N-linked carbohydrate composition of these proteins were examined and compared. Vinblastine- or colchicine-selected cells made a 125-kDa precursor that was rapidly processed (t( 1/2 ) ≃ 20 min) to mature forms of 135 and 140 kDa, respectively. Heterogeneity between the 135- and 140-kDa forms of the molecule can be attributed to N-linked carbohydrate. In contrast, taxol-selected cells made two precursors, 125 and 120 kDa, which appeared within 5 and 15 min after the onset of pulse labeling, respectively. They were processed to mature forms of 140 and 130 kDa. Since a single deglycosylated precursor or mature form was not observed after enzymatic removal of N-linked oligosaccharides, other differences, besides N-linked glycosylation, which occur in early processing compartments, are likely to account for the two multidrug resistance-associated glycoproteins in taxol-selected cells. These results demonstrate that a family of multidrug resistance-associated glycoproteins can be differentially expressed.

Original languageEnglish (US)
Pages (from-to)13685-13689
Number of pages5
JournalJournal of Biological Chemistry
Volume262
Issue number28
StatePublished - 1987

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Biosynthesis
Multiple Drug Resistance
Glycoproteins
Paclitaxel
Vinblastine
Colchicine
Carbohydrates
Glycosylation
Electrophoretic mobility
Macrophages
Membrane Glycoproteins
Cell membranes
Oligosaccharides
Labeling
Cell Membrane
Phenotype
Molecules
Processing
Chemical analysis
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Biosynthesis of heterogeneous forms of multidrug resistance-associated glycoproteins. / Greenberger, L. M.; Williams, S. S.; Band Horwitz, Susan.

In: Journal of Biological Chemistry, Vol. 262, No. 28, 1987, p. 13685-13689.

Research output: Contribution to journalArticle

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