Biosynthesis, maturation, and acid activation of the semliki forest virus fusion protein

Margaret Kielian, Steven Jungerwirth, Kathleen Ullrich Sayad, Susan Decandido

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The Semliki Forest virus spike protein has a potent membrane fusion activity which is activated in vivo by the low pH of endocytic vacuoles. The spike protein is composed of two transmembrane subunits, E1 and E2, plus E3, a peripheral polypeptide. Acid-induced conformational changes in the E1 or E2 subunits were analyzed by using monoclonal antibodies specific for the acid-treated spike protein. E1 and E2 reacted with the antibodies after treatment of wild-type or mutant virus at the pH of fusion. The E1 conformational change resembled fusion in its requirement for both low pH and cholesterol. Pulse-chase analysis and intracellular pH treatment were then used to determine the ability of the newly synthesized spike to undergo acid-induced conformational changes. p62, the precursor to E2 and E3, was shown to undergo a pH-dependent conformational change similar to that of E2 and was sensitive to acid very soon after biosynthesis. In contrast, a posttranslational maturation event was required for the conversion of E1 to the pH-sensitive form. E1 maturation occurred fairly late in the exocytic pathway, after the virus spike had passed the medial Golgi but before incorporation of the spike into a new virus particle.

Original languageEnglish (US)
Pages (from-to)4614-4624
Number of pages11
JournalJournal of Virology
Volume64
Issue number10
StatePublished - 1990

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viral fusion proteins
Semliki Forest virus
Viral Fusion Proteins
Semliki forest virus
biosynthesis
Acids
acids
Viruses
viruses
Proteins
Membrane Fusion
proteins
Vacuoles
virion
Virion
vacuoles
polypeptides
monoclonal antibodies
Cholesterol
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Kielian, M., Jungerwirth, S., Sayad, K. U., & Decandido, S. (1990). Biosynthesis, maturation, and acid activation of the semliki forest virus fusion protein. Journal of Virology, 64(10), 4614-4624.

Biosynthesis, maturation, and acid activation of the semliki forest virus fusion protein. / Kielian, Margaret; Jungerwirth, Steven; Sayad, Kathleen Ullrich; Decandido, Susan.

In: Journal of Virology, Vol. 64, No. 10, 1990, p. 4614-4624.

Research output: Contribution to journalArticle

Kielian, M, Jungerwirth, S, Sayad, KU & Decandido, S 1990, 'Biosynthesis, maturation, and acid activation of the semliki forest virus fusion protein', Journal of Virology, vol. 64, no. 10, pp. 4614-4624.
Kielian, Margaret ; Jungerwirth, Steven ; Sayad, Kathleen Ullrich ; Decandido, Susan. / Biosynthesis, maturation, and acid activation of the semliki forest virus fusion protein. In: Journal of Virology. 1990 ; Vol. 64, No. 10. pp. 4614-4624.
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