Biosynthesis and intra-axonal transport of proteins during neuronal regeneration

D. Giulian, H. Des Ruisseaux, David Cowburn

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Intraocular injections of radiolabeled amino acids permitted the study of protein biosynthesis in goldfish retinal ganglion cells and the distribution of this material to the synapse via intra-axonal transport, during regeneration of the fish visual system. There is a 3-fold increase in amino acid incorporated within the ganglion cell layer compared to intact contralateral controls by 10 days after axotomy. Tubulin and actin are the major proteins synthesized and axonally transported. In addition, glycosylation of proteins is also increased, with the appearance of three fucosylated polypeptides in regenerating optic fibers not detected in controls. The peak of amino acid incorporation is a 5-fold increase which occurs about 30 days after cutting and which is paralleled by increased amounts of axonally transported proteins to the optic fibers. Growing axons reach the optic tectum 30 days after transection, carrying newly synthesized protein towards the site of synapse formation. A minor polypeptide of high molecular weight is synthesized by regenerating ganglion cells and is slowly transported axonally to the optic tectum. It can be detected only within regenerating neurons during later stages of growth. Amino acid incorporation and axonal transport return to near control levels after the 50th day, approximately when recovery of sight occurs. Overall, retinal ganglion cells show significant increases in the biosynthesis, in the intra-axonal transport, and in the glycosylation of proteins during regeneration. Major synthetic events are enhanced production of structural proteins, which persist throughout the regrowth process. Other synthetic events include the production of minor high molecular weight polypeptides which are not detected within the intact visual system.

Original languageEnglish (US)
Pages (from-to)6494-6501
Number of pages8
JournalJournal of Biological Chemistry
Volume255
Issue number13
StatePublished - 1980
Externally publishedYes

Fingerprint

Axonal Transport
Biosynthesis
Regeneration
Carrier Proteins
Amino Acids
Superior Colliculi
Retinal Ganglion Cells
Glycosylation
Ganglia
Synapses
Peptides
Proteins
Molecular Weight
Intraocular Injections
Axotomy
Goldfish
Protein Biosynthesis
Fiber optics
Tubulin
Optics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Biosynthesis and intra-axonal transport of proteins during neuronal regeneration. / Giulian, D.; Des Ruisseaux, H.; Cowburn, David.

In: Journal of Biological Chemistry, Vol. 255, No. 13, 1980, p. 6494-6501.

Research output: Contribution to journalArticle

@article{27421c4470f84d41922c87d3a8186424,
title = "Biosynthesis and intra-axonal transport of proteins during neuronal regeneration",
abstract = "Intraocular injections of radiolabeled amino acids permitted the study of protein biosynthesis in goldfish retinal ganglion cells and the distribution of this material to the synapse via intra-axonal transport, during regeneration of the fish visual system. There is a 3-fold increase in amino acid incorporated within the ganglion cell layer compared to intact contralateral controls by 10 days after axotomy. Tubulin and actin are the major proteins synthesized and axonally transported. In addition, glycosylation of proteins is also increased, with the appearance of three fucosylated polypeptides in regenerating optic fibers not detected in controls. The peak of amino acid incorporation is a 5-fold increase which occurs about 30 days after cutting and which is paralleled by increased amounts of axonally transported proteins to the optic fibers. Growing axons reach the optic tectum 30 days after transection, carrying newly synthesized protein towards the site of synapse formation. A minor polypeptide of high molecular weight is synthesized by regenerating ganglion cells and is slowly transported axonally to the optic tectum. It can be detected only within regenerating neurons during later stages of growth. Amino acid incorporation and axonal transport return to near control levels after the 50th day, approximately when recovery of sight occurs. Overall, retinal ganglion cells show significant increases in the biosynthesis, in the intra-axonal transport, and in the glycosylation of proteins during regeneration. Major synthetic events are enhanced production of structural proteins, which persist throughout the regrowth process. Other synthetic events include the production of minor high molecular weight polypeptides which are not detected within the intact visual system.",
author = "D. Giulian and {Des Ruisseaux}, H. and David Cowburn",
year = "1980",
language = "English (US)",
volume = "255",
pages = "6494--6501",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "13",

}

TY - JOUR

T1 - Biosynthesis and intra-axonal transport of proteins during neuronal regeneration

AU - Giulian, D.

AU - Des Ruisseaux, H.

AU - Cowburn, David

PY - 1980

Y1 - 1980

N2 - Intraocular injections of radiolabeled amino acids permitted the study of protein biosynthesis in goldfish retinal ganglion cells and the distribution of this material to the synapse via intra-axonal transport, during regeneration of the fish visual system. There is a 3-fold increase in amino acid incorporated within the ganglion cell layer compared to intact contralateral controls by 10 days after axotomy. Tubulin and actin are the major proteins synthesized and axonally transported. In addition, glycosylation of proteins is also increased, with the appearance of three fucosylated polypeptides in regenerating optic fibers not detected in controls. The peak of amino acid incorporation is a 5-fold increase which occurs about 30 days after cutting and which is paralleled by increased amounts of axonally transported proteins to the optic fibers. Growing axons reach the optic tectum 30 days after transection, carrying newly synthesized protein towards the site of synapse formation. A minor polypeptide of high molecular weight is synthesized by regenerating ganglion cells and is slowly transported axonally to the optic tectum. It can be detected only within regenerating neurons during later stages of growth. Amino acid incorporation and axonal transport return to near control levels after the 50th day, approximately when recovery of sight occurs. Overall, retinal ganglion cells show significant increases in the biosynthesis, in the intra-axonal transport, and in the glycosylation of proteins during regeneration. Major synthetic events are enhanced production of structural proteins, which persist throughout the regrowth process. Other synthetic events include the production of minor high molecular weight polypeptides which are not detected within the intact visual system.

AB - Intraocular injections of radiolabeled amino acids permitted the study of protein biosynthesis in goldfish retinal ganglion cells and the distribution of this material to the synapse via intra-axonal transport, during regeneration of the fish visual system. There is a 3-fold increase in amino acid incorporated within the ganglion cell layer compared to intact contralateral controls by 10 days after axotomy. Tubulin and actin are the major proteins synthesized and axonally transported. In addition, glycosylation of proteins is also increased, with the appearance of three fucosylated polypeptides in regenerating optic fibers not detected in controls. The peak of amino acid incorporation is a 5-fold increase which occurs about 30 days after cutting and which is paralleled by increased amounts of axonally transported proteins to the optic fibers. Growing axons reach the optic tectum 30 days after transection, carrying newly synthesized protein towards the site of synapse formation. A minor polypeptide of high molecular weight is synthesized by regenerating ganglion cells and is slowly transported axonally to the optic tectum. It can be detected only within regenerating neurons during later stages of growth. Amino acid incorporation and axonal transport return to near control levels after the 50th day, approximately when recovery of sight occurs. Overall, retinal ganglion cells show significant increases in the biosynthesis, in the intra-axonal transport, and in the glycosylation of proteins during regeneration. Major synthetic events are enhanced production of structural proteins, which persist throughout the regrowth process. Other synthetic events include the production of minor high molecular weight polypeptides which are not detected within the intact visual system.

UR - http://www.scopus.com/inward/record.url?scp=0019159234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019159234&partnerID=8YFLogxK

M3 - Article

C2 - 6156161

AN - SCOPUS:0019159234

VL - 255

SP - 6494

EP - 6501

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 13

ER -