Biosensors for characterizing the dynamics of Rho family GTPases in living cells

Louis Hodgson, Feimo Shen, Klaus Hahn

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

The biosensors developed in the authors laboratory have been based on different designs, each imparting specific strengths and weaknesses. Here we describe detailed protocols for the application of three biosensors exemplifying different designsfirst, a design in which an environmentally sensitive dye is used to report the activation of endogenous Cdc42, followed by two biosensors based on FRET, one using intramolecular and the other intermolecular FRET. The design differences lead to the need for different approaches in imaging and image analysis. Curr. Protoc. Cell Biol. 46:14.11.1-14.11.26.

Original languageEnglish (US)
Pages (from-to)14111-141126
Number of pages127016
JournalCurrent Protocols in Cell Biology
Issue numberSUPPLO. 46
DOIs
StatePublished - 2010

Keywords

  • Biosensors
  • FRET
  • Live cell imaging
  • Rho family GTPases

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Biosensors for characterizing the dynamics of Rho family GTPases in living cells'. Together they form a unique fingerprint.

Cite this