Abstract
The biosensors developed in the authors laboratory have been based on different designs, each imparting specific strengths and weaknesses. Here we describe detailed protocols for the application of three biosensors exemplifying different designsfirst, a design in which an environmentally sensitive dye is used to report the activation of endogenous Cdc42, followed by two biosensors based on FRET, one using intramolecular and the other intermolecular FRET. The design differences lead to the need for different approaches in imaging and image analysis. Curr. Protoc. Cell Biol. 46:14.11.1-14.11.26.
Original language | English (US) |
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Pages (from-to) | 14111-141126 |
Number of pages | 127016 |
Journal | Current Protocols in Cell Biology |
Issue number | SUPPLO. 46 |
DOIs | |
State | Published - 2010 |
Keywords
- Biosensors
- FRET
- Live cell imaging
- Rho family GTPases
ASJC Scopus subject areas
- Cell Biology