Biosensors for characterizing the dynamics of Rho family GTPases in living cells

Louis Hodgson, Feimo Shen, Klaus Hahn

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The biosensors developed in the authors laboratory have been based on different designs, each imparting specific strengths and weaknesses. Here we describe detailed protocols for the application of three biosensors exemplifying different designsfirst, a design in which an environmentally sensitive dye is used to report the activation of endogenous Cdc42, followed by two biosensors based on FRET, one using intramolecular and the other intermolecular FRET. The design differences lead to the need for different approaches in imaging and image analysis. Curr. Protoc. Cell Biol. 46:14.11.1-14.11.26.

Original languageEnglish (US)
Pages (from-to)14111-141126
Number of pages127016
JournalCurrent Protocols in Cell Biology
Issue numberSUPPLO. 46
DOIs
StatePublished - 2010

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rho GTP-Binding Proteins
Family Relations
Biosensing Techniques
Coloring Agents

Keywords

  • Biosensors
  • FRET
  • Live cell imaging
  • Rho family GTPases

ASJC Scopus subject areas

  • Cell Biology

Cite this

Biosensors for characterizing the dynamics of Rho family GTPases in living cells. / Hodgson, Louis; Shen, Feimo; Hahn, Klaus.

In: Current Protocols in Cell Biology, No. SUPPLO. 46, 2010, p. 14111-141126.

Research output: Contribution to journalArticle

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