Biomechanical and structural response of healing Achilles tendon to fatigue loading following acute injury

Benjamin R. Freedman, Joseph J. Sarver, Mark R. Buckley, Pramod B. Voleti, Louis J. Soslowsky

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Achilles tendon injuries affect both athletes and the general population, and their incidence is rising. In particular, the Achilles tendon is subject to dynamic loading at or near failure loads during activity, and fatigue induced damage is likely a contributing factor to ultimate tendon failure. Unfortunately, little is known about how injured Achilles tendons respond mechanically and structurally to fatigue loading during healing. Knowledge of these properties remains critical to best evaluate tendon damage induction and the ability of the tendon to maintain mechanical properties with repeated loading. Thus, this study investigated the mechanical and structural changes in healing mouse Achilles tendons during fatigue loading. Twenty four mice received bilateral full thickness, partial width excisional injuries to their Achilles tendons (IACUC approved) and twelve tendons from six uninjured mice were used as controls. Tendons were fatigue loaded to assess mechanical and structural properties simultaneously after 0, 1, 3, and 6 weeks of healing using an integrated polarized light system. Results showed that the number of cycles to failure decreased dramatically (37-fold, p<0.005) due to injury, but increased throughout healing, ultimately recovering after 6 weeks. The tangent stiffness, hysteresis, and dynamic modulus did not improve with healing (p<0.005). Linear regression analysis was used to determine relationships between mechanical and structural properties. Of tendon structural properties, the apparent birefringence was able to best predict dynamic modulus (R2=0.88-0.92) throughout healing and fatigue life. This study reinforces the concept that fatigue loading is a sensitive metric to assess tendon healing and demonstrates potential structural metrics to predict mechanical properties.

Original languageEnglish (US)
Pages (from-to)2028-2034
Number of pages7
JournalJournal of Biomechanics
Volume47
Issue number9
DOIs
StatePublished - Jun 27 2014
Externally publishedYes

Fingerprint

Achilles Tendon
Tendons
Fatigue
Fatigue of materials
Wounds and Injuries
Structural properties
Animal Care Committees
Mechanical properties
Tendon Injuries
Birefringence
Athletes
Linear Models
Regression Analysis
Light
Light polarization
Linear regression
Regression analysis
Incidence
Hysteresis
Population

Keywords

  • Achilles tendon
  • Damage
  • Fatigue mechanics
  • Foot and ankle
  • Healing
  • Imaging
  • Injury

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Rehabilitation
  • Biophysics
  • Biomedical Engineering

Cite this

Biomechanical and structural response of healing Achilles tendon to fatigue loading following acute injury. / Freedman, Benjamin R.; Sarver, Joseph J.; Buckley, Mark R.; Voleti, Pramod B.; Soslowsky, Louis J.

In: Journal of Biomechanics, Vol. 47, No. 9, 27.06.2014, p. 2028-2034.

Research output: Contribution to journalArticle

Freedman, Benjamin R. ; Sarver, Joseph J. ; Buckley, Mark R. ; Voleti, Pramod B. ; Soslowsky, Louis J. / Biomechanical and structural response of healing Achilles tendon to fatigue loading following acute injury. In: Journal of Biomechanics. 2014 ; Vol. 47, No. 9. pp. 2028-2034.
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