TY - JOUR
T1 - Biological systems analysis at molecular and subcellular levels
T2 - chromosome positioning inside human nuclei
AU - Sun, Hui Bin
AU - Shen, Jin
AU - El-Mounayri, Omar
AU - Yokota, Hiroki
PY - 1998
Y1 - 1998
N2 - A systems mechanical analysis of biological samples, particularly DNA molecules and synthetic nuclei was carried out. Using optical, fluorescence, and atomic force microscopy, individual molecular and subcellular images of biological samples were detected. The mechanical properties of DNA molecules with and without bound-proteins were studied by applying small forces induced by surface tension or viscous drag and stretched DNA molecules. DNA molecules, modeled as random coil in solution, were straightened and immobilized on flat surface, and a stress-strain relationship was analyzed. Preliminary result suggests that the DNA molecules with bound-proteins behave differently in solution from the DNA molecules alone. By monitoring a process of nucleus formation, a linkage of a shape and a size of nuclei to a state of DNA and a stress field in a nucleus suspension was observed.
AB - A systems mechanical analysis of biological samples, particularly DNA molecules and synthetic nuclei was carried out. Using optical, fluorescence, and atomic force microscopy, individual molecular and subcellular images of biological samples were detected. The mechanical properties of DNA molecules with and without bound-proteins were studied by applying small forces induced by surface tension or viscous drag and stretched DNA molecules. DNA molecules, modeled as random coil in solution, were straightened and immobilized on flat surface, and a stress-strain relationship was analyzed. Preliminary result suggests that the DNA molecules with bound-proteins behave differently in solution from the DNA molecules alone. By monitoring a process of nucleus formation, a linkage of a shape and a size of nuclei to a state of DNA and a stress field in a nucleus suspension was observed.
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M3 - Article
AN - SCOPUS:0032256452
SN - 0278-940X
VL - 26
SP - 402
EP - 403
JO - Critical Reviews in Biomedical Engineering
JF - Critical Reviews in Biomedical Engineering
IS - 5
ER -