Biological significance of cancer-associated sialyl-Tn antigen

Modulation of malignant phenotype in gastric carcinoma cells

Sandra I. Pinho, Nuno T. Marcos, Bibiana Ferreira, Ana S. Carvalho, Maria J. Oliveira, Filipe Santos-Silva, Anne Harduin-Lepers, Celso A. Reis

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

The activation of an abnormal glycosylation pathway in cancer cells leads to the formation of the sialyl-Tn antigen, blocking regular carbohydrate chain elongation. Sialyl-Tn antigen is rarely expressed in normal tissues but is aberrantly expressed in a variety of carcinomas, where it constitutes a marker of poor prognosis. Although the clinical significance of sialyl-Tn is well characterized, a functional role for this glycan and its contribution to cancer progression remain to be elucidated. This study evaluates the capability of sialyl-Tn to modify processes like cell cycle, apoptosis, actin cytoskeleton dynamics, adhesion and motility on ECM components, cell-cell aggregation and invasion. De-novo expression of sialyl-Tn leads to major morphological and cell behavior alterations in gastric carcinoma cells which were reverted by specific antibody blockage. Sialyl-Tn antigen is able to modulate a malignant phenotype inducing a more aggressive cell behavior, such as decreased cell-cell aggregation and increased ECM adhesion, migration and invasion.

Original languageEnglish (US)
Pages (from-to)157-170
Number of pages14
JournalCancer Letters
Volume249
Issue number2
DOIs
StatePublished - May 8 2007
Externally publishedYes

Fingerprint

Stomach
Carcinoma
Phenotype
Cell Aggregation
Neoplasms
Cellular Structures
Actin Cytoskeleton
Glycosylation
Polysaccharides
Cell Cycle
Carbohydrates
sialosyl-Tn antigen
Apoptosis
Antibodies

Keywords

  • Adhesion
  • Gastric carcinoma
  • Invasion
  • Malignant phenotype
  • Sialyl-Tn
  • Sialyltransferase

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Biological significance of cancer-associated sialyl-Tn antigen : Modulation of malignant phenotype in gastric carcinoma cells. / Pinho, Sandra I.; Marcos, Nuno T.; Ferreira, Bibiana; Carvalho, Ana S.; Oliveira, Maria J.; Santos-Silva, Filipe; Harduin-Lepers, Anne; Reis, Celso A.

In: Cancer Letters, Vol. 249, No. 2, 08.05.2007, p. 157-170.

Research output: Contribution to journalArticle

Pinho, SI, Marcos, NT, Ferreira, B, Carvalho, AS, Oliveira, MJ, Santos-Silva, F, Harduin-Lepers, A & Reis, CA 2007, 'Biological significance of cancer-associated sialyl-Tn antigen: Modulation of malignant phenotype in gastric carcinoma cells', Cancer Letters, vol. 249, no. 2, pp. 157-170. https://doi.org/10.1016/j.canlet.2006.08.010
Pinho, Sandra I. ; Marcos, Nuno T. ; Ferreira, Bibiana ; Carvalho, Ana S. ; Oliveira, Maria J. ; Santos-Silva, Filipe ; Harduin-Lepers, Anne ; Reis, Celso A. / Biological significance of cancer-associated sialyl-Tn antigen : Modulation of malignant phenotype in gastric carcinoma cells. In: Cancer Letters. 2007 ; Vol. 249, No. 2. pp. 157-170.
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