Biological alchemy

Engineering bone and fat from fat-derived stem cells

James A. Lee, Brian M. Parrett, Jorge A. Conejero, Jordan Laser, John Chen, Amy J. Kogon, Dawne Nanda, Robert T. Grant, Arnold S. Breitbart, Christopher Godek, Joseph E. Losee

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Adipose tissue contains a population of pluripotent stem cells capable of differentiating along multiple mesenchymal cell lineages. In this study the authors isolated these fat-derived stem cells successfully from Lewis rats and induced differentiation along adipogenic and osteogenic lineages in vitro and in vivo. Induction was stimulated by exposing stem cells to lineage-specific induction factors. Adipocyte-inducing media contained dexamethasone, insulin, and isobutyl-methylxanthine. Osteoblast inducing media contained dexamethasone, β-glycerophosphate, and ascorbic acid. Undifferentiated stem cells were maintained in minimal essential media alpha and fetal bovine serum. At 10 days, cells cultured in adipogenic media differentiated into adipocytes in vitro, as evidenced by positive Oil red 0 staining of lipid vacuoles. At 21 days, cells cultured in osteogenic media differentiated into osteoblasts in vitro as demonstrated by Alizarin red staining of a calcified extracellular matrix and immunohisto-chemical staining for osteocalcin. Differentiated cells were seeded at a density of 5 × 106 cells onto 15 × 15-mm polyglycolic acid grafts and implanted subcutaneously into three groups of Lewis rats: Group I contained undifferentiated stem cell grafts, group II contained adipocyte grafts, and group III contained osteoblast grafts. At weeks 4 and 8, in vivo fat formation was demonstrated in group II rats, as confirmed by Oil red O staining. At 8 weeks, group III rats demonstrated in vivo bone formation, as confirmed by the presence of osteocalcin on immunohistochemistry and the characteristic morphology of bone on hematoxylin-eosin staining. Group I rats demonstrated no in vivo bone or fat formation at either time interval. These results demonstrate the ability to isolate pluripotent stem cells from adipose tissue, to induce their differentiation into osteoblasts and adipocytes in vitro, and to form bone and fat subsequently in vivo. This is the first published report of in vivo bone formation from fat-derived stem cells. These cells may eventually serve as a readily available source of autologous stem cells for the engineering of bone and fat.

Original languageEnglish (US)
Pages (from-to)610-617
Number of pages8
JournalAnnals of Plastic Surgery
Volume50
Issue number6
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

Fingerprint

Alchemy
Bioengineering
Stem Cells
Fats
Osteoblasts
Adipocytes
Bone and Bones
Staining and Labeling
Transplants
Pluripotent Stem Cells
Osteocalcin
Cell Lineage
Osteogenesis
Dexamethasone
Adipose Tissue
Cultured Cells
Polyglycolic Acid
Cell Engineering
Glycerophosphates
Hematoxylin

ASJC Scopus subject areas

  • Surgery

Cite this

Biological alchemy : Engineering bone and fat from fat-derived stem cells. / Lee, James A.; Parrett, Brian M.; Conejero, Jorge A.; Laser, Jordan; Chen, John; Kogon, Amy J.; Nanda, Dawne; Grant, Robert T.; Breitbart, Arnold S.; Godek, Christopher; Losee, Joseph E.

In: Annals of Plastic Surgery, Vol. 50, No. 6, 01.06.2003, p. 610-617.

Research output: Contribution to journalArticle

Lee, JA, Parrett, BM, Conejero, JA, Laser, J, Chen, J, Kogon, AJ, Nanda, D, Grant, RT, Breitbart, AS, Godek, C & Losee, JE 2003, 'Biological alchemy: Engineering bone and fat from fat-derived stem cells', Annals of Plastic Surgery, vol. 50, no. 6, pp. 610-617. https://doi.org/10.1097/01.SAP.0000069069.23266.35
Lee, James A. ; Parrett, Brian M. ; Conejero, Jorge A. ; Laser, Jordan ; Chen, John ; Kogon, Amy J. ; Nanda, Dawne ; Grant, Robert T. ; Breitbart, Arnold S. ; Godek, Christopher ; Losee, Joseph E. / Biological alchemy : Engineering bone and fat from fat-derived stem cells. In: Annals of Plastic Surgery. 2003 ; Vol. 50, No. 6. pp. 610-617.
@article{ca4afd1efbd24d64aa9ab01c6bfbdc7e,
title = "Biological alchemy: Engineering bone and fat from fat-derived stem cells",
abstract = "Adipose tissue contains a population of pluripotent stem cells capable of differentiating along multiple mesenchymal cell lineages. In this study the authors isolated these fat-derived stem cells successfully from Lewis rats and induced differentiation along adipogenic and osteogenic lineages in vitro and in vivo. Induction was stimulated by exposing stem cells to lineage-specific induction factors. Adipocyte-inducing media contained dexamethasone, insulin, and isobutyl-methylxanthine. Osteoblast inducing media contained dexamethasone, β-glycerophosphate, and ascorbic acid. Undifferentiated stem cells were maintained in minimal essential media alpha and fetal bovine serum. At 10 days, cells cultured in adipogenic media differentiated into adipocytes in vitro, as evidenced by positive Oil red 0 staining of lipid vacuoles. At 21 days, cells cultured in osteogenic media differentiated into osteoblasts in vitro as demonstrated by Alizarin red staining of a calcified extracellular matrix and immunohisto-chemical staining for osteocalcin. Differentiated cells were seeded at a density of 5 × 106 cells onto 15 × 15-mm polyglycolic acid grafts and implanted subcutaneously into three groups of Lewis rats: Group I contained undifferentiated stem cell grafts, group II contained adipocyte grafts, and group III contained osteoblast grafts. At weeks 4 and 8, in vivo fat formation was demonstrated in group II rats, as confirmed by Oil red O staining. At 8 weeks, group III rats demonstrated in vivo bone formation, as confirmed by the presence of osteocalcin on immunohistochemistry and the characteristic morphology of bone on hematoxylin-eosin staining. Group I rats demonstrated no in vivo bone or fat formation at either time interval. These results demonstrate the ability to isolate pluripotent stem cells from adipose tissue, to induce their differentiation into osteoblasts and adipocytes in vitro, and to form bone and fat subsequently in vivo. This is the first published report of in vivo bone formation from fat-derived stem cells. These cells may eventually serve as a readily available source of autologous stem cells for the engineering of bone and fat.",
author = "Lee, {James A.} and Parrett, {Brian M.} and Conejero, {Jorge A.} and Jordan Laser and John Chen and Kogon, {Amy J.} and Dawne Nanda and Grant, {Robert T.} and Breitbart, {Arnold S.} and Christopher Godek and Losee, {Joseph E.}",
year = "2003",
month = "6",
day = "1",
doi = "10.1097/01.SAP.0000069069.23266.35",
language = "English (US)",
volume = "50",
pages = "610--617",
journal = "Annals of Plastic Surgery",
issn = "0148-7043",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Biological alchemy

T2 - Engineering bone and fat from fat-derived stem cells

AU - Lee, James A.

AU - Parrett, Brian M.

AU - Conejero, Jorge A.

AU - Laser, Jordan

AU - Chen, John

AU - Kogon, Amy J.

AU - Nanda, Dawne

AU - Grant, Robert T.

AU - Breitbart, Arnold S.

AU - Godek, Christopher

AU - Losee, Joseph E.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - Adipose tissue contains a population of pluripotent stem cells capable of differentiating along multiple mesenchymal cell lineages. In this study the authors isolated these fat-derived stem cells successfully from Lewis rats and induced differentiation along adipogenic and osteogenic lineages in vitro and in vivo. Induction was stimulated by exposing stem cells to lineage-specific induction factors. Adipocyte-inducing media contained dexamethasone, insulin, and isobutyl-methylxanthine. Osteoblast inducing media contained dexamethasone, β-glycerophosphate, and ascorbic acid. Undifferentiated stem cells were maintained in minimal essential media alpha and fetal bovine serum. At 10 days, cells cultured in adipogenic media differentiated into adipocytes in vitro, as evidenced by positive Oil red 0 staining of lipid vacuoles. At 21 days, cells cultured in osteogenic media differentiated into osteoblasts in vitro as demonstrated by Alizarin red staining of a calcified extracellular matrix and immunohisto-chemical staining for osteocalcin. Differentiated cells were seeded at a density of 5 × 106 cells onto 15 × 15-mm polyglycolic acid grafts and implanted subcutaneously into three groups of Lewis rats: Group I contained undifferentiated stem cell grafts, group II contained adipocyte grafts, and group III contained osteoblast grafts. At weeks 4 and 8, in vivo fat formation was demonstrated in group II rats, as confirmed by Oil red O staining. At 8 weeks, group III rats demonstrated in vivo bone formation, as confirmed by the presence of osteocalcin on immunohistochemistry and the characteristic morphology of bone on hematoxylin-eosin staining. Group I rats demonstrated no in vivo bone or fat formation at either time interval. These results demonstrate the ability to isolate pluripotent stem cells from adipose tissue, to induce their differentiation into osteoblasts and adipocytes in vitro, and to form bone and fat subsequently in vivo. This is the first published report of in vivo bone formation from fat-derived stem cells. These cells may eventually serve as a readily available source of autologous stem cells for the engineering of bone and fat.

AB - Adipose tissue contains a population of pluripotent stem cells capable of differentiating along multiple mesenchymal cell lineages. In this study the authors isolated these fat-derived stem cells successfully from Lewis rats and induced differentiation along adipogenic and osteogenic lineages in vitro and in vivo. Induction was stimulated by exposing stem cells to lineage-specific induction factors. Adipocyte-inducing media contained dexamethasone, insulin, and isobutyl-methylxanthine. Osteoblast inducing media contained dexamethasone, β-glycerophosphate, and ascorbic acid. Undifferentiated stem cells were maintained in minimal essential media alpha and fetal bovine serum. At 10 days, cells cultured in adipogenic media differentiated into adipocytes in vitro, as evidenced by positive Oil red 0 staining of lipid vacuoles. At 21 days, cells cultured in osteogenic media differentiated into osteoblasts in vitro as demonstrated by Alizarin red staining of a calcified extracellular matrix and immunohisto-chemical staining for osteocalcin. Differentiated cells were seeded at a density of 5 × 106 cells onto 15 × 15-mm polyglycolic acid grafts and implanted subcutaneously into three groups of Lewis rats: Group I contained undifferentiated stem cell grafts, group II contained adipocyte grafts, and group III contained osteoblast grafts. At weeks 4 and 8, in vivo fat formation was demonstrated in group II rats, as confirmed by Oil red O staining. At 8 weeks, group III rats demonstrated in vivo bone formation, as confirmed by the presence of osteocalcin on immunohistochemistry and the characteristic morphology of bone on hematoxylin-eosin staining. Group I rats demonstrated no in vivo bone or fat formation at either time interval. These results demonstrate the ability to isolate pluripotent stem cells from adipose tissue, to induce their differentiation into osteoblasts and adipocytes in vitro, and to form bone and fat subsequently in vivo. This is the first published report of in vivo bone formation from fat-derived stem cells. These cells may eventually serve as a readily available source of autologous stem cells for the engineering of bone and fat.

UR - http://www.scopus.com/inward/record.url?scp=0037607332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037607332&partnerID=8YFLogxK

U2 - 10.1097/01.SAP.0000069069.23266.35

DO - 10.1097/01.SAP.0000069069.23266.35

M3 - Article

VL - 50

SP - 610

EP - 617

JO - Annals of Plastic Surgery

JF - Annals of Plastic Surgery

SN - 0148-7043

IS - 6

ER -