Biocompatibility of quantum dots (CdSe/ZnS) in human amniotic membrane-derived mesenchymal stem cells in vitro

Gongping Wang, Guangwei Zeng, Caie Wang, Huasheng Wang, Bo Yang, Fangxia Guan, Dongpeng Li, Xiaoshan Feng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background and Aim. Amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) are a potential source of mesenchymal stem cells which could be used to repair skin damage. The use of mesenchymal stem cells to repair skin damage requires safe, effective and biocompatible agents to evaluate the effectiveness of the result. Quantum dots (QDs) composed of CdSe/ZnS are semiconductor nanocrystals with broad excitation and narrow emission spectra, which have been considered as a new chemical and fluorescent substance for non-invasively labeling different cells in vitro and in vivo. This study investigated the cytotoxic effects of QDs on hAM-dMSCs at different times following labeling. Methods. Using 0.75, 1.5 and 3.0 μL between quantum dots, labeled human amniotic mesenchymal stem cells were collected on days 1, 2 and 4 and observed morphological changes, performed an MTT cell growth assay and flow cytometry for mesenchymal stem cells molecular markers. Results. Quantum dot concentration 0.75 μg/mL labeled under a fluorescence microscope, cell morphology was observed, The MTT assay showed cells in the proliferative phase. Flow cytometry expression CD29, CD31, CD34, CD44, CD90, CD105 and CD106. Conclusions. Within a certain range of concentrations between quantum dots labeled human amniotic mesenchymal stem cells has good biocompatibility.

Original languageEnglish (US)
Pages (from-to)227-233
Number of pages7
JournalBiomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
Volume159
Issue number2
DOIs
StatePublished - Jul 1 2015
Externally publishedYes

Fingerprint

Quantum Dots
Amnion
Stem cells
Mesenchymal Stromal Cells
Biocompatibility
Semiconductor quantum dots
Membranes
Flow cytometry
Labeling
Assays
Skin
Flow Cytometry
Repair
Cell growth
Nanocrystals
In Vitro Techniques
Microscopes
Fluorescence
Semiconductor materials
Growth

Keywords

  • Biocompatibility
  • Human amniotic membrane
  • Mesenchymal stem cells
  • Quantum dots

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Biocompatibility of quantum dots (CdSe/ZnS) in human amniotic membrane-derived mesenchymal stem cells in vitro. / Wang, Gongping; Zeng, Guangwei; Wang, Caie; Wang, Huasheng; Yang, Bo; Guan, Fangxia; Li, Dongpeng; Feng, Xiaoshan.

In: Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia, Vol. 159, No. 2, 01.07.2015, p. 227-233.

Research output: Contribution to journalArticle

Wang, Gongping ; Zeng, Guangwei ; Wang, Caie ; Wang, Huasheng ; Yang, Bo ; Guan, Fangxia ; Li, Dongpeng ; Feng, Xiaoshan. / Biocompatibility of quantum dots (CdSe/ZnS) in human amniotic membrane-derived mesenchymal stem cells in vitro. In: Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia. 2015 ; Vol. 159, No. 2. pp. 227-233.
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