Biochemical mechanisms of generation bradykinin by endotoxin

R. L. Miller, Michael J. Reichgott, K. L. Melmon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The vasodilatory properties of bradykinin are compatible with a pathogenetic role in man and subhuman primates during endotoxemia. The peptide is generated during endotoxemia when peripheral vascular resistance is decreased. A number of mechanisms of kinin generation can be recruited by endotoxin. In plasma containing complement and 19S antibody to endotoxin, the polysaccharide moiety of endotoxin activates plasma kallikreins. Similar quantities of bradykinin are generated in all species tested. Therefore, species related variation in production of the peptide in vivo depends on the interaction of endotoxin with other tissues. Granulocytes of primates (as opposed to those of rabbits) contain a cytoplasmic kallikrein or kallikrein activator. Granulocytes release their cytoplasmic enzymes (and presumably kallikreins) when phagocytizing endotoxin. In vitro phagocytosis requires complement and lipid rich endotoxin. Polysaccharide fractions of endotoxin do not produce effects on granulocytes, their kinin generation, or the cardiovascular system. It is suggested that differences in endotoxin effect among species are largely related to effects of the lipid moiety of endotoxin and the chemical machinery of the cells this moiety penetrates.

Original languageEnglish (US)
JournalJournal of Infectious Diseases
VolumeSp16
Issue number128
StatePublished - 1973
Externally publishedYes

Fingerprint

Bradykinin
Endotoxins
Kallikreins
Granulocytes
Kinins
Endotoxemia
Vascular Resistance
Primates
Polysaccharides
Plasma Kallikrein
Lipids
Peptides
Cardiovascular System
Phagocytosis
Rabbits
Antibodies
Enzymes

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Biochemical mechanisms of generation bradykinin by endotoxin. / Miller, R. L.; Reichgott, Michael J.; Melmon, K. L.

In: Journal of Infectious Diseases, Vol. Sp16, No. 128, 1973.

Research output: Contribution to journalArticle

@article{c10b0dba0cf044c1866b2706c7336c4d,
title = "Biochemical mechanisms of generation bradykinin by endotoxin",
abstract = "The vasodilatory properties of bradykinin are compatible with a pathogenetic role in man and subhuman primates during endotoxemia. The peptide is generated during endotoxemia when peripheral vascular resistance is decreased. A number of mechanisms of kinin generation can be recruited by endotoxin. In plasma containing complement and 19S antibody to endotoxin, the polysaccharide moiety of endotoxin activates plasma kallikreins. Similar quantities of bradykinin are generated in all species tested. Therefore, species related variation in production of the peptide in vivo depends on the interaction of endotoxin with other tissues. Granulocytes of primates (as opposed to those of rabbits) contain a cytoplasmic kallikrein or kallikrein activator. Granulocytes release their cytoplasmic enzymes (and presumably kallikreins) when phagocytizing endotoxin. In vitro phagocytosis requires complement and lipid rich endotoxin. Polysaccharide fractions of endotoxin do not produce effects on granulocytes, their kinin generation, or the cardiovascular system. It is suggested that differences in endotoxin effect among species are largely related to effects of the lipid moiety of endotoxin and the chemical machinery of the cells this moiety penetrates.",
author = "Miller, {R. L.} and Reichgott, {Michael J.} and Melmon, {K. L.}",
year = "1973",
language = "English (US)",
volume = "Sp16",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "128",

}

TY - JOUR

T1 - Biochemical mechanisms of generation bradykinin by endotoxin

AU - Miller, R. L.

AU - Reichgott, Michael J.

AU - Melmon, K. L.

PY - 1973

Y1 - 1973

N2 - The vasodilatory properties of bradykinin are compatible with a pathogenetic role in man and subhuman primates during endotoxemia. The peptide is generated during endotoxemia when peripheral vascular resistance is decreased. A number of mechanisms of kinin generation can be recruited by endotoxin. In plasma containing complement and 19S antibody to endotoxin, the polysaccharide moiety of endotoxin activates plasma kallikreins. Similar quantities of bradykinin are generated in all species tested. Therefore, species related variation in production of the peptide in vivo depends on the interaction of endotoxin with other tissues. Granulocytes of primates (as opposed to those of rabbits) contain a cytoplasmic kallikrein or kallikrein activator. Granulocytes release their cytoplasmic enzymes (and presumably kallikreins) when phagocytizing endotoxin. In vitro phagocytosis requires complement and lipid rich endotoxin. Polysaccharide fractions of endotoxin do not produce effects on granulocytes, their kinin generation, or the cardiovascular system. It is suggested that differences in endotoxin effect among species are largely related to effects of the lipid moiety of endotoxin and the chemical machinery of the cells this moiety penetrates.

AB - The vasodilatory properties of bradykinin are compatible with a pathogenetic role in man and subhuman primates during endotoxemia. The peptide is generated during endotoxemia when peripheral vascular resistance is decreased. A number of mechanisms of kinin generation can be recruited by endotoxin. In plasma containing complement and 19S antibody to endotoxin, the polysaccharide moiety of endotoxin activates plasma kallikreins. Similar quantities of bradykinin are generated in all species tested. Therefore, species related variation in production of the peptide in vivo depends on the interaction of endotoxin with other tissues. Granulocytes of primates (as opposed to those of rabbits) contain a cytoplasmic kallikrein or kallikrein activator. Granulocytes release their cytoplasmic enzymes (and presumably kallikreins) when phagocytizing endotoxin. In vitro phagocytosis requires complement and lipid rich endotoxin. Polysaccharide fractions of endotoxin do not produce effects on granulocytes, their kinin generation, or the cardiovascular system. It is suggested that differences in endotoxin effect among species are largely related to effects of the lipid moiety of endotoxin and the chemical machinery of the cells this moiety penetrates.

UR - http://www.scopus.com/inward/record.url?scp=0015642484&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015642484&partnerID=8YFLogxK

M3 - Article

C2 - 4719682

AN - SCOPUS:0015642484

VL - Sp16

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 128

ER -