Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte

Michael B. Prystowsky, J. Ely, S. N. Vogel, E. Goldwasser, F. W. Fitch

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Mls-reactive murine helper T cell clone L2 produces at least 10 lymphokine activities affecting at least five distinct target cells. Culture conditions can be optimized to enable production of high levels of colony-stimulating factors (CSFs) by lectin stimulation of L2 cells under serum-free conditions. Selective enrichment of three lymphokine activities (interleukin 2, CSF, and interferon) can be achieved by using a combination of phenyl-Sepharose and hydroxyapatite chromatography. At least two types of CSF can be separated by concanavalin A-Sepharose chromatography. The CSF that does not bind to concanavalin A-Sepharose can be enriched to a specific activity of at least 5 x 108 U/mg of protein by using ion exchange high-pressure liquid chromatography.

Original languageEnglish (US)
Pages (from-to)2757-2761
Number of pages5
JournalFederation Proceedings
Volume42
Issue number10
StatePublished - 1983
Externally publishedYes

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Colony-Stimulating Factors
Lymphokines
Helper-Inducer T-Lymphocytes
Agarose Chromatography
Ion Exchange
Durapatite
Lectins
Interferons
Interleukin-2
Clone Cells
High Pressure Liquid Chromatography
Serum
Proteins
concanavalin A-sepharose

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Prystowsky, M. B., Ely, J., Vogel, S. N., Goldwasser, E., & Fitch, F. W. (1983). Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte. Federation Proceedings, 42(10), 2757-2761.

Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte. / Prystowsky, Michael B.; Ely, J.; Vogel, S. N.; Goldwasser, E.; Fitch, F. W.

In: Federation Proceedings, Vol. 42, No. 10, 1983, p. 2757-2761.

Research output: Contribution to journalArticle

Prystowsky, MB, Ely, J, Vogel, SN, Goldwasser, E & Fitch, FW 1983, 'Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte', Federation Proceedings, vol. 42, no. 10, pp. 2757-2761.
Prystowsky, Michael B. ; Ely, J. ; Vogel, S. N. ; Goldwasser, E. ; Fitch, F. W. / Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte. In: Federation Proceedings. 1983 ; Vol. 42, No. 10. pp. 2757-2761.
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