Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte

M. B. Prystowsky, J. Ely, S. N. Vogel, E. Goldwasser, F. W. Fitch

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Abstract

The Mls-reactive murine helper T cell clone L2 produces at least 10 lymphokine activities affecting at least five distinct target cells. Culture conditions can be optimized to enable production of high levels of colony-stimulating factors (CSFs) by lectin stimulation of L2 cells under serum-free conditions. Selective enrichment of three lymphokine activities (interleukin 2, CSF, and interferon) can be achieved by using a combination of phenyl-Sepharose and hydroxyapatite chromatography. At least two types of CSF can be separated by concanavalin A-Sepharose chromatography. The CSF that does not bind to concanavalin A-Sepharose can be enriched to a specific activity of at least 5 x 108 U/mg of protein by using ion exchange high-pressure liquid chromatography.

Original languageEnglish (US)
Pages (from-to)2757-2761
Number of pages5
JournalFederation Proceedings
Volume42
Issue number10
Publication statusPublished - Oct 21 1983
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Prystowsky, M. B., Ely, J., Vogel, S. N., Goldwasser, E., & Fitch, F. W. (1983). Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte. Federation Proceedings, 42(10), 2757-2761.