TY - JOUR
T1 - Biochemical and genetic characterization of the multidrug resistance phenotype in murine macrophage-like J774.2 cells
AU - Kirschner, Lawrence S.
AU - Greenberger, Lee M.
AU - Hsu, Stephen I.Hong
AU - Yang, Chia Ping Huang
AU - Cohen, Dalia
AU - Piekarz, Richard L.
AU - Castillo, Gonzalo
AU - Han, Edward Kyu Ho
AU - Yu, Lijia
AU - Horwitz, Susan Band
PY - 1992/1/9
Y1 - 1992/1/9
N2 - The development of multidrug resistance (MDR) in malignant tumors is a major obstacle to the treatment of many cancers. MDR sublines have been derived from the J774.2 mouse macrophage-like cell line and utilized to characterize the phenotype at the biochemical and genetic level. Two isoforms of the drug resistance-associated P-glycoprotein are present and distinguishable both electrophoretically and pharmacologically. Genetic analysis has revealed the presence of a three-member gene family; expression of two of these genes, mdr1a and mdr1b, is associated with MDR whereas the expression of the third, mdr2, is not. Studies of these three genes have revealed similarities and differences in the manner in which they are regulated at the transcriptional level, and have suggested that post-transcriptional effects may also be important.
AB - The development of multidrug resistance (MDR) in malignant tumors is a major obstacle to the treatment of many cancers. MDR sublines have been derived from the J774.2 mouse macrophage-like cell line and utilized to characterize the phenotype at the biochemical and genetic level. Two isoforms of the drug resistance-associated P-glycoprotein are present and distinguishable both electrophoretically and pharmacologically. Genetic analysis has revealed the presence of a three-member gene family; expression of two of these genes, mdr1a and mdr1b, is associated with MDR whereas the expression of the third, mdr2, is not. Studies of these three genes have revealed similarities and differences in the manner in which they are regulated at the transcriptional level, and have suggested that post-transcriptional effects may also be important.
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U2 - 10.1016/0006-2952(92)90664-5
DO - 10.1016/0006-2952(92)90664-5
M3 - Article
C2 - 1346495
AN - SCOPUS:0026547001
SN - 0006-2952
VL - 43
SP - 77
EP - 87
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 1
ER -