Biochemical and genetic characterization of the multidrug resistance phenotype in murine macrophage-like J774.2 cells

Lawrence S. Kirschner, Lee M. Greenberger, Stephen I.Hong Hsu, Chia Ping Huang Yang, Dalia Cohen, Richard L. Piekarz, Gonzalo Castillo, Edward Kyu Ho Han, Lijia Yu, Susan Band Horwitz

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The development of multidrug resistance (MDR) in malignant tumors is a major obstacle to the treatment of many cancers. MDR sublines have been derived from the J774.2 mouse macrophage-like cell line and utilized to characterize the phenotype at the biochemical and genetic level. Two isoforms of the drug resistance-associated P-glycoprotein are present and distinguishable both electrophoretically and pharmacologically. Genetic analysis has revealed the presence of a three-member gene family; expression of two of these genes, mdr1a and mdr1b, is associated with MDR whereas the expression of the third, mdr2, is not. Studies of these three genes have revealed similarities and differences in the manner in which they are regulated at the transcriptional level, and have suggested that post-transcriptional effects may also be important.

Original languageEnglish (US)
Pages (from-to)77-87
Number of pages11
JournalBiochemical Pharmacology
Volume43
Issue number1
DOIs
StatePublished - Jan 9 1992

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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