Binding of [125i]wheat germ agglutinin to chinese hamster ovary cells under conditions which affect the mobility of membrane components

Pamela Stanley, Jeremy P. Carver

Research output: Contribution to journalArticle

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Abstract

The binding of [125I]wheat germ agglutinin ([12I]WGA) of high specific activity to Chinese hamster ovary (CHO) cells has been examined over a millionfold range of WGA concentrations and correlated with the phenomena of agglutination and capping by WGA. Analysis of the binding data by the method of Scatchard gives a complex curve indicative of positive cooperativity amongst high-affinity binding sites. Binding assays performed under conditions which inhibit capping and/or agglutination, such as low temperature or glutaraldehyde fixation, give similarly complex binding curves. Thus, the gross mobility of WGA receptors in the membrane does not appear to be responsible for the cooperative binding of WGA to CHO cells.

Original languageEnglish (US)
Pages (from-to)617-622
Number of pages6
JournalJournal of Cell Biology
Volume79
Issue number3
DOIs
StatePublished - Dec 1 1978

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Keywords

  • Membrane mobility
  • Scatchard plots
  • Wheat germ agglutinin

ASJC Scopus subject areas

  • Cell Biology

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