Binding of NO and CO to the d1 heme of cd1 nitrite reductase from Pseudomonas aeruginosa

T. K. Das, E. K. Wilson, F. Cutruzzolà, M. Brunori, Denis L. Rousseau

Research output: Contribution to journalArticle

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Abstract

The cd1 nitrite reductase, a key enzyme in bacterial denitrification, catalyzes the one-electron reduction of nitrite to nitric oxide. The enzyme contains two redox centers, a c-type heine and a unique d1 heme, which is a dioxoisobacteriochlorin. Nitric oxide, generated by this enzymatic pathway, if not removed from the medium, can bind to the ferrous d1 cofactor with extremely high affinity and inhibit enzyme activity. In this paper, we report the resonance Raman investigation of the properties of nitric oxide and carbon monoxide binding to the d1 site of the reduced enzyme. The Fe-ligand (Fe-NO and Fe-CO) stretching vibrational frequencies are unusually high in comparison to those of other ferrous heine complexes. The frequencies of the Fe-NO and N-O stretching modes appear at 585 and 1626 cm-1, respectively, in the NO complex, while the frequencies of the Fe-CO and C-O stretching modes are at 563 and 1972 cm-1, respectively, for the CO complex. Also, the widths (fwhm) of the Fe-CO and C-O stretching modes are smaller than those observed in the corresponding complexes of other heine proteins. The unusual spectroscopic characteristics of the d1 cofactor are discussed in terms of both its unique electronic properties and the strongly polar distal environment around the iron-bound ligand. It is likely that the influence of a highly ruffled structure of heme d1 on its electronic properties is the major factor causing anomalous Fe-ligand vibrational frequencies.

Original languageEnglish (US)
Pages (from-to)10774-10781
Number of pages8
JournalBiochemistry
Volume40
Issue number36
DOIs
StatePublished - Sep 11 2001

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Nitrite Reductases
Carbon Monoxide
Pseudomonas aeruginosa
Stretching
Nitric Oxide
Vibrational spectra
Enzymes
Ligands
Electronic properties
Denitrification
Enzyme activity
Nitrites
Oxidation-Reduction
Iron
heme d1
nitrite reductase (heme)
Electrons
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Binding of NO and CO to the d1 heme of cd1 nitrite reductase from Pseudomonas aeruginosa. / Das, T. K.; Wilson, E. K.; Cutruzzolà, F.; Brunori, M.; Rousseau, Denis L.

In: Biochemistry, Vol. 40, No. 36, 11.09.2001, p. 10774-10781.

Research output: Contribution to journalArticle

Das, T. K. ; Wilson, E. K. ; Cutruzzolà, F. ; Brunori, M. ; Rousseau, Denis L. / Binding of NO and CO to the d1 heme of cd1 nitrite reductase from Pseudomonas aeruginosa. In: Biochemistry. 2001 ; Vol. 40, No. 36. pp. 10774-10781.
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