Binding of carbon nanotube to BMP receptor 2 enhances cell differentiation and inhibits apoptosis via regulating bHLH transcription factors

Y. Zhang; Q. Mu; H. Zhou; K. Vrijens; M. F. Roussel; G. Jiang; B. Yan

doi:10.1038/cddis.2012.48

Binding of carbon nanotube to BMP receptor 2 enhances cell differentiation and inhibits apoptosis via regulating bHLH transcription factors

Y. Zhang, Q. Mu, H. Zhou, K. Vrijens, M. F. Roussel, G. Jiang, B. Yan

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Biomaterials that can drive stem cells to an appropriate differentiation level and decrease apoptosis of transplanted cells are needed in regenerative medicine. Nanomaterials are promising novel materials for such applications. Here we reported that carboxylated multiwalled carbon nanotube (MWCNT 1) promotes myogenic differentiation of mouse myoblast cells and inhibits cell apoptosis under the differentiation conditions by regulating basic helix-loop-helix transcription factors. MWCNT 1 attenuates bone morphogenetic protein receptor (BMPR) signaling activity by binding to BMPR2 and attenuating the phosphorylation of BMPR1. This molecular understanding allowed us to tune stem cell differentiation to various levels by chemical modifications, demonstrating human control of biological activities of nanoparticles and opening an avenue for potential applications of nanomaterials in regenerative medicine.

Original language	English (US)
Article number	e308
Journal	Cell Death and Disease
Volume	3
Issue number	5
DOIs	https://doi.org/10.1038/cddis.2012.48
State	Published - May 24 2012
Externally published	Yes

Keywords

BMP signaling
Carbon nanotube
Cell differentiation
Membrane receptor
Regenerative medicine

ASJC Scopus subject areas

Immunology
Cellular and Molecular Neuroscience
Cell Biology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/cddis.2012.48

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title = "Binding of carbon nanotube to BMP receptor 2 enhances cell differentiation and inhibits apoptosis via regulating bHLH transcription factors",

abstract = "Biomaterials that can drive stem cells to an appropriate differentiation level and decrease apoptosis of transplanted cells are needed in regenerative medicine. Nanomaterials are promising novel materials for such applications. Here we reported that carboxylated multiwalled carbon nanotube (MWCNT 1) promotes myogenic differentiation of mouse myoblast cells and inhibits cell apoptosis under the differentiation conditions by regulating basic helix-loop-helix transcription factors. MWCNT 1 attenuates bone morphogenetic protein receptor (BMPR) signaling activity by binding to BMPR2 and attenuating the phosphorylation of BMPR1. This molecular understanding allowed us to tune stem cell differentiation to various levels by chemical modifications, demonstrating human control of biological activities of nanoparticles and opening an avenue for potential applications of nanomaterials in regenerative medicine.",

keywords = "BMP signaling, Carbon nanotube, Cell differentiation, Membrane receptor, Regenerative medicine",

author = "Y. Zhang and Q. Mu and H. Zhou and K. Vrijens and Roussel, {M. F.} and G. Jiang and B. Yan",

note = "Funding Information: Acknowledgements. We thank Fu-yue Zeng, Victoria Frohlich, Yannan Ouyang, Sharon Frase, Linda Mann and Jianmin Ye for their technical assistance and Paul B Yu (Harvard Medical School), Michael A Rudnicki (Ottawa Health Research Institute) and Carme Gallego (Universitat de Lleida) for providing constitutive active ALK2 and ALK3 constructs, myogenin-luciferase and p21-luciferase constructs. This work was supported by the National Basic Research Program of China (2010CB933504), National Natural Science Foundation of China (21077068 and 21137002), National Cancer Institute (P30CA027165), NIH (CA096832 and CA21765 for MFR) and the American Lebanese Syrian Associated Charities.",

year = "2012",

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day = "24",

doi = "10.1038/cddis.2012.48",

language = "English (US)",

volume = "3",

journal = "Cell Death and Disease",

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T1 - Binding of carbon nanotube to BMP receptor 2 enhances cell differentiation and inhibits apoptosis via regulating bHLH transcription factors

AU - Zhang, Y.

AU - Mu, Q.

AU - Zhou, H.

AU - Vrijens, K.

AU - Roussel, M. F.

AU - Jiang, G.

AU - Yan, B.

N1 - Funding Information: Acknowledgements. We thank Fu-yue Zeng, Victoria Frohlich, Yannan Ouyang, Sharon Frase, Linda Mann and Jianmin Ye for their technical assistance and Paul B Yu (Harvard Medical School), Michael A Rudnicki (Ottawa Health Research Institute) and Carme Gallego (Universitat de Lleida) for providing constitutive active ALK2 and ALK3 constructs, myogenin-luciferase and p21-luciferase constructs. This work was supported by the National Basic Research Program of China (2010CB933504), National Natural Science Foundation of China (21077068 and 21137002), National Cancer Institute (P30CA027165), NIH (CA096832 and CA21765 for MFR) and the American Lebanese Syrian Associated Charities.

PY - 2012/5/24

Y1 - 2012/5/24

N2 - Biomaterials that can drive stem cells to an appropriate differentiation level and decrease apoptosis of transplanted cells are needed in regenerative medicine. Nanomaterials are promising novel materials for such applications. Here we reported that carboxylated multiwalled carbon nanotube (MWCNT 1) promotes myogenic differentiation of mouse myoblast cells and inhibits cell apoptosis under the differentiation conditions by regulating basic helix-loop-helix transcription factors. MWCNT 1 attenuates bone morphogenetic protein receptor (BMPR) signaling activity by binding to BMPR2 and attenuating the phosphorylation of BMPR1. This molecular understanding allowed us to tune stem cell differentiation to various levels by chemical modifications, demonstrating human control of biological activities of nanoparticles and opening an avenue for potential applications of nanomaterials in regenerative medicine.

AB - Biomaterials that can drive stem cells to an appropriate differentiation level and decrease apoptosis of transplanted cells are needed in regenerative medicine. Nanomaterials are promising novel materials for such applications. Here we reported that carboxylated multiwalled carbon nanotube (MWCNT 1) promotes myogenic differentiation of mouse myoblast cells and inhibits cell apoptosis under the differentiation conditions by regulating basic helix-loop-helix transcription factors. MWCNT 1 attenuates bone morphogenetic protein receptor (BMPR) signaling activity by binding to BMPR2 and attenuating the phosphorylation of BMPR1. This molecular understanding allowed us to tune stem cell differentiation to various levels by chemical modifications, demonstrating human control of biological activities of nanoparticles and opening an avenue for potential applications of nanomaterials in regenerative medicine.

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KW - Carbon nanotube

KW - Cell differentiation

KW - Membrane receptor

KW - Regenerative medicine

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Binding of carbon nanotube to BMP receptor 2 enhances cell differentiation and inhibits apoptosis via regulating bHLH transcription factors

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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