Binding of aggregated γ globulin to activated T lymphocytes in the guinea pig

J. Van Boxel, David L. Rosenstreich

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Heat aggregated guinea pig γ globulin was shown to bind to the surface membrane of a subclass of guinea pig T lymphocytes. Cells of this subpopulation were identified as T lymphocytes because these cells did not stain for surface Ig (a B cell marker) but did form spontaneous E rosettes with rabbit erythrocytes (a T cell marker). A strikingly high proportion of such aggregate binding (Agg+), E rosette forming (E rosette+), but surface Ig negative (Ig-) cells were found in an inflammatory exudate. Thus purified peritoneal exudate lymphocytes (PELs) are known to consist of over 90% T cells, and 59% of these cells bound aggregates. 10% of these Agg+ Ig- E rosette+ cells were found in draining lymph node cell populations and none in thymus cell populations. The high frequency among PELs suggested that these Aggregate+ Ig- E rosette+ cells might be activated T cells as these are known to occur in high proportion in PEL populations. Confirmatory evidence for this postulate was provided by the striking increase (from 10% to 46%) of Ig- E rosette+ cells that bound aggregates when lymph node cells were activated by antigen stimulation in vitro.

Original languageEnglish (US)
Pages (from-to)1002-1012
Number of pages11
JournalJournal of Experimental Medicine
Volume139
Issue number4
StatePublished - 1974
Externally publishedYes

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Globulins
Guinea Pigs
T-Lymphocytes
Exudates and Transudates
Lymphocytes
Lymph Nodes
Population
Thymus Gland
B-Lymphocytes
Coloring Agents
Hot Temperature
Erythrocytes
Rabbits
Antigens
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Binding of aggregated γ globulin to activated T lymphocytes in the guinea pig. / Van Boxel, J.; Rosenstreich, David L.

In: Journal of Experimental Medicine, Vol. 139, No. 4, 1974, p. 1002-1012.

Research output: Contribution to journalArticle

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