Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5

Ganjam V. Kalpana, Shana Marmon, Weidong Wang, Gerald R. Crabtree, Stephen P. Goff

Research output: Contribution to journalArticle

431 Citations (Scopus)

Abstract

Upon entry into a host cell, retroviruses direct the reverse transcription of the viral RNA genome and the establishment of an integrated proviral DNA. The retroviral integrase protein (IN) is responsible for the insertion of the viral DNA into host chromosomal targets. The two-hybrid system was used to identify a human gene product that binds tightly to the human immunodeficiency virus-type 1 (HIV-1) integrase in vitro and stimulates its DNA-joining activity. The sequence of the gene suggests that the protein is a human homolog of yeast SNF5, a transcriptional activator required for high-level expression of many genes. The gene, termed INI1 (for integrase interactor 1), may encode a nuclear factor that promotes integration and targets incoming viral DNA to active genes.

Original languageEnglish (US)
Pages (from-to)2002-2006
Number of pages5
JournalScience
Volume266
Issue number5193
StatePublished - 1994
Externally publishedYes

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Integrases
HIV-1
Transcription Factors
Yeasts
Viral DNA
Genes
Viral Genome
DNA
Viral RNA
Retroviridae
Reverse Transcription
Proteins
Gene Expression

ASJC Scopus subject areas

  • General

Cite this

Kalpana, G. V., Marmon, S., Wang, W., Crabtree, G. R., & Goff, S. P. (1994). Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5. Science, 266(5193), 2002-2006.

Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5. / Kalpana, Ganjam V.; Marmon, Shana; Wang, Weidong; Crabtree, Gerald R.; Goff, Stephen P.

In: Science, Vol. 266, No. 5193, 1994, p. 2002-2006.

Research output: Contribution to journalArticle

Kalpana, GV, Marmon, S, Wang, W, Crabtree, GR & Goff, SP 1994, 'Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5', Science, vol. 266, no. 5193, pp. 2002-2006.
Kalpana, Ganjam V. ; Marmon, Shana ; Wang, Weidong ; Crabtree, Gerald R. ; Goff, Stephen P. / Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5. In: Science. 1994 ; Vol. 266, No. 5193. pp. 2002-2006.
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