Bilirubin conjugates produced by human, dog, and rat hepatocytes transplanted into athymic Gunn rats.

P. Lahiri, P. R. Yerneni, Z. Khan, A. A. Demetriou, A. D. Moscioni, D. Shouval, Jayanta Roy-Chowdhury, N. R. Chowdhury

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Abstract

We developed a new hybrid mutant rat (athymic Gunn hybrid strain) that does not form and excrete conjugated bilirubin into bile and accepts xenografts because of T-lymphocyte deficiency. Cryopreserved isolated normal human, dog, or rat liver cells attached to collagen-coated microcarriers were transplanted into the athymic Gunn hybrid rats. Transplantation of human or rat liver cells resulted in biliary excretion of bilirubin conjugates predominantly as glucuronides, whereas isolated dog liver cells likewise transplanted resulted in the excretion of 40% to 50% of the conjugates as a glucoside-glucuronide diconjugate. The biliary bilirubin conjugates of the transplanted athymic Gunn hybrid rats paralleled those of the donor species. The data indicate that the types of bilirubin conjugates excreted by each species are determined by intrinsic properties of the respective hepatocytes rather than nutritional or other environmental factors. The findings also show that conjugation of bilirubin after liver cell transplantation results from functioning of the engrafted cells rather than activation of endogenous uridine diphosphatelucuronosyltransferase activity of the host.

Original languageEnglish (US)
Pages (from-to)58-63
Number of pages6
JournalJournal of the Association for Academic Minority Physicians : the official publication of the Association for Academic Minority Physicians
Volume2
Issue number2
StatePublished - 1991

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Gunn Rats
Nude Rats
Bilirubin
Hepatocytes
Dogs
Glucuronides
Liver
Uridine
Cell Transplantation
Glucosides
Heterografts
Bile
Liver Transplantation
Collagen
Transplantation
T-Lymphocytes

ASJC Scopus subject areas

  • Medicine(all)

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Bilirubin conjugates produced by human, dog, and rat hepatocytes transplanted into athymic Gunn rats. / Lahiri, P.; Yerneni, P. R.; Khan, Z.; Demetriou, A. A.; Moscioni, A. D.; Shouval, D.; Roy-Chowdhury, Jayanta; Chowdhury, N. R.

In: Journal of the Association for Academic Minority Physicians : the official publication of the Association for Academic Minority Physicians, Vol. 2, No. 2, 1991, p. 58-63.

Research output: Contribution to journalArticle

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AU - Khan, Z.

AU - Demetriou, A. A.

AU - Moscioni, A. D.

AU - Shouval, D.

AU - Roy-Chowdhury, Jayanta

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