Beta-Barrel Scaffold of Fluorescent Proteins. Folding, Stability and Role in Chromophore Formation.

Olesya V. Stepanenko, Olga V. Stepanenko, Irina M. Kuznetsova, Vladislav Verkhusha, Konstantin K. Turoverov

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

This review focuses on the current view of the interaction between the β-barrel scaffold of fluorescent proteins and their unique chromophore located in the internal helix. The chromophore originates from the polypeptide chain and its properties are. influenced by the surrounding protein matrix of the β-barrel. On the other hand, it appears that a chromophore tightens the β-barrel scaffold and plays a crucial role in its stability. Furthermore, the presence of a mature chromophore causes hysteresis of protein unfolding and refolding. We survey studies measuring protein unfolding and refolding using traditional methods as well as new approaches, such as mechanical unfolding and reassembly of truncated fluorescent proteins. We also analyze models of fluorescent protein unfolding and refolding obtained through different approaches, and compare the results of protein folding in vitro to co-translational folding of a newly synthesized polypeptide chain.

Original languageEnglish (US)
Pages (from-to)221-278
Number of pages58
JournalInternational Review of Cell and Molecular Biology
Volume302
DOIs
StatePublished - 2013

Fingerprint

Protein Refolding
Protein Unfolding
Chromophores
Scaffolds
Peptides
Proteins
Protein Folding
Protein folding
Hysteresis

Keywords

  • β-barrel scaffold
  • Chromophore and protein interaction
  • Fluorescence proteins
  • Hysteresis
  • Protein folding
  • RFP
  • Split GFP
  • Superfolder GFP

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Cite this

Beta-Barrel Scaffold of Fluorescent Proteins. Folding, Stability and Role in Chromophore Formation. / Stepanenko, Olesya V.; Stepanenko, Olga V.; Kuznetsova, Irina M.; Verkhusha, Vladislav; Turoverov, Konstantin K.

In: International Review of Cell and Molecular Biology, Vol. 302, 2013, p. 221-278.

Research output: Contribution to journalArticle

Stepanenko, Olesya V. ; Stepanenko, Olga V. ; Kuznetsova, Irina M. ; Verkhusha, Vladislav ; Turoverov, Konstantin K. / Beta-Barrel Scaffold of Fluorescent Proteins. Folding, Stability and Role in Chromophore Formation. In: International Review of Cell and Molecular Biology. 2013 ; Vol. 302. pp. 221-278.
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