Bending of the estrogen response element by polyamines and estrogen receptors α and β: A fluorescence resonance energy transfer study

Veena Vijayanathan, T. J. Thomas, Sandhya K. Nair, Akira Shirahata, Michael A. Gallo, Thresia Thomas

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Estrogenic regulation of gene expression is mediated by the binding of the hormone to its receptors (ERα and ERβ) followed by their binding to estrogen response element (ERE). Previous studies showed that natural polyamines - putrescine, spermidine, and spermine - facilitated ERα·ERE recognition. We determined the effects of natural and synthetic polyamines on the bending of a 27-mer oligonucleotide (ODN) harboring the ERE (ERE-ODN), using fluorescence resonance energy transfer (FRET) technique. Complementary strands of the ERE-ODN were labeled with fluorescein and tetramethylrhodamine, as donor and acceptor, respectively. The ERE-ODN was intrinsically bent with an end-to-end distance of 76 ± 2 Å, compared to a theoretical value of 98 Å. The end-to-end distance of the ERE-ODN was reduced to 64 Å in the presence of 250 μM spermine. A control ODN with scrambled sequence did not show intrinsic bending or spermine-induced bending. Alkyl substitution at the pendant amino groups reduced the ability of spermine to bend the ERE-ODN. Both ERα and ERβ decreased the end-to-end distance of the ERE-ODN, although ERα was more efficient than ERβ in inducing ERE bending. Spermine-induced bending of the ERE-ODN was significantly increased by ERα. Fluorescence anisotropy measurement showed that the equilibrium association constant of ERα-ERE binding increased by 12-fold in the presence of 250 μM spermine compared to control. The free energy change (ΔG) of ERα·ERE complex formation was -13.1 kcal/mol at 22 °C in the presence of spermine. Our results suggest that polyamine-induced bending of the ERE might be a mechanism for enhancing ERα-ERE binding affinity and thereby fine-tuning the transcriptional response of estrogen-responsive genes.

Original languageEnglish (US)
Pages (from-to)1191-1206
Number of pages16
JournalInternational Journal of Biochemistry and Cell Biology
Volume38
Issue number7
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Fluorescence Resonance Energy Transfer
Polyamines
Response Elements
Estrogen Receptors
Estrogens
Spermine
Fluorescence Polarization
Putrescine
Spermidine
Gene Expression Regulation
Fluorescein
Gene expression
Oligonucleotides
Free energy

Keywords

  • DNA bending
  • Estrogen receptor
  • Fluorescence resonance energy transfer
  • Spermine

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Bending of the estrogen response element by polyamines and estrogen receptors α and β : A fluorescence resonance energy transfer study. / Vijayanathan, Veena; Thomas, T. J.; Nair, Sandhya K.; Shirahata, Akira; Gallo, Michael A.; Thomas, Thresia.

In: International Journal of Biochemistry and Cell Biology, Vol. 38, No. 7, 2006, p. 1191-1206.

Research output: Contribution to journalArticle

Vijayanathan, Veena ; Thomas, T. J. ; Nair, Sandhya K. ; Shirahata, Akira ; Gallo, Michael A. ; Thomas, Thresia. / Bending of the estrogen response element by polyamines and estrogen receptors α and β : A fluorescence resonance energy transfer study. In: International Journal of Biochemistry and Cell Biology. 2006 ; Vol. 38, No. 7. pp. 1191-1206.
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AU - Shirahata, Akira

AU - Gallo, Michael A.

AU - Thomas, Thresia

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AB - Estrogenic regulation of gene expression is mediated by the binding of the hormone to its receptors (ERα and ERβ) followed by their binding to estrogen response element (ERE). Previous studies showed that natural polyamines - putrescine, spermidine, and spermine - facilitated ERα·ERE recognition. We determined the effects of natural and synthetic polyamines on the bending of a 27-mer oligonucleotide (ODN) harboring the ERE (ERE-ODN), using fluorescence resonance energy transfer (FRET) technique. Complementary strands of the ERE-ODN were labeled with fluorescein and tetramethylrhodamine, as donor and acceptor, respectively. The ERE-ODN was intrinsically bent with an end-to-end distance of 76 ± 2 Å, compared to a theoretical value of 98 Å. The end-to-end distance of the ERE-ODN was reduced to 64 Å in the presence of 250 μM spermine. A control ODN with scrambled sequence did not show intrinsic bending or spermine-induced bending. Alkyl substitution at the pendant amino groups reduced the ability of spermine to bend the ERE-ODN. Both ERα and ERβ decreased the end-to-end distance of the ERE-ODN, although ERα was more efficient than ERβ in inducing ERE bending. Spermine-induced bending of the ERE-ODN was significantly increased by ERα. Fluorescence anisotropy measurement showed that the equilibrium association constant of ERα-ERE binding increased by 12-fold in the presence of 250 μM spermine compared to control. The free energy change (ΔG) of ERα·ERE complex formation was -13.1 kcal/mol at 22 °C in the presence of spermine. Our results suggest that polyamine-induced bending of the ERE might be a mechanism for enhancing ERα-ERE binding affinity and thereby fine-tuning the transcriptional response of estrogen-responsive genes.

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