BCL-6 protein is expressed in germinal-center B cells

G. Cattoretti, C. C. Chang, K. Cechova, J. Zhang, B. Hilda Ye, B. Falini, D. C. Louie, K. Offit, R. S K Chaganti, R. Dalla-Favera

Research output: Contribution to journalArticle

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Abstract

Structural alterations of the 5' noncoding region of the BCL-6 gene have been found in 40% of diffuse large cell lymphoma (DLCL) and 5% to 10% of follicular lymphomas (FL), suggesting that deregulated BCL-6 expression may play a role in lymphomagenesis. Nucleotide sequencing of BCL-6 cDNA predicted a protein containing six zinc-finger domains, suggesting that it may function as a transcription factor. Using antisera raised against N- and C-terminal BCL-6 synthetic oligopeptides in immunoprecipitation, immunoblot, and immunocytochemical assays, this study identifies the BCL-6 gene product as a 95-kD nuclear protein. Western blot analysis of human tumor cell lines representative of various hematopoietic lineages/stages of differentiation showed that the BCL-6 protein is predominantly expressed in the B-cell lineage where it was found in mature B cells. Immunohistochemical analysis of normal human lymphoid tissues indicated that BCL-6 expression is topographically restricted to germinal centers including all centroblasts and centrocytes. The BCL-6 protein was also detectable in inter- and intrafollicular CD4+ T cells, but not in other follicular components including mantle-zone B cells, plasma cells, dendritic cells, and macrophages. Immunohistochemical analysis of DLCL and FL biopsy samples showed that the BCL-6 protein is detectable in these tumors independent of the presence of BCL-6 gene rearrangements. These results indicate that the expression of the BCL-6 gene is specifically regulated during B-cell differentiation and suggest a role for BCL-6 in germinal center development or function. Because DLCL derive from germinal-center B cells, deregulated BCL-6 expression may contribute to lymphomagenesis by preventing postgerminal center differentiation.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalBlood
Volume86
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

Germinal Center
B-Lymphocytes
Cells
Lymphoma, Large B-Cell, Diffuse
Genes
Follicular Lymphoma
Proteins
Tumors
Oligopeptides
Gene Rearrangement
Zinc Fingers
Lymphoid Tissue
Cell Lineage
Nuclear Proteins
Plasma Cells
Tumor Cell Line
T-cells
Immunoprecipitation
Biopsy
Macrophages

ASJC Scopus subject areas

  • Hematology

Cite this

Cattoretti, G., Chang, C. C., Cechova, K., Zhang, J., Ye, B. H., Falini, B., ... Dalla-Favera, R. (1995). BCL-6 protein is expressed in germinal-center B cells. Blood, 86(1), 45-53.

BCL-6 protein is expressed in germinal-center B cells. / Cattoretti, G.; Chang, C. C.; Cechova, K.; Zhang, J.; Ye, B. Hilda; Falini, B.; Louie, D. C.; Offit, K.; Chaganti, R. S K; Dalla-Favera, R.

In: Blood, Vol. 86, No. 1, 1995, p. 45-53.

Research output: Contribution to journalArticle

Cattoretti, G, Chang, CC, Cechova, K, Zhang, J, Ye, BH, Falini, B, Louie, DC, Offit, K, Chaganti, RSK & Dalla-Favera, R 1995, 'BCL-6 protein is expressed in germinal-center B cells', Blood, vol. 86, no. 1, pp. 45-53.
Cattoretti G, Chang CC, Cechova K, Zhang J, Ye BH, Falini B et al. BCL-6 protein is expressed in germinal-center B cells. Blood. 1995;86(1):45-53.
Cattoretti, G. ; Chang, C. C. ; Cechova, K. ; Zhang, J. ; Ye, B. Hilda ; Falini, B. ; Louie, D. C. ; Offit, K. ; Chaganti, R. S K ; Dalla-Favera, R. / BCL-6 protein is expressed in germinal-center B cells. In: Blood. 1995 ; Vol. 86, No. 1. pp. 45-53.
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abstract = "Structural alterations of the 5' noncoding region of the BCL-6 gene have been found in 40{\%} of diffuse large cell lymphoma (DLCL) and 5{\%} to 10{\%} of follicular lymphomas (FL), suggesting that deregulated BCL-6 expression may play a role in lymphomagenesis. Nucleotide sequencing of BCL-6 cDNA predicted a protein containing six zinc-finger domains, suggesting that it may function as a transcription factor. Using antisera raised against N- and C-terminal BCL-6 synthetic oligopeptides in immunoprecipitation, immunoblot, and immunocytochemical assays, this study identifies the BCL-6 gene product as a 95-kD nuclear protein. Western blot analysis of human tumor cell lines representative of various hematopoietic lineages/stages of differentiation showed that the BCL-6 protein is predominantly expressed in the B-cell lineage where it was found in mature B cells. Immunohistochemical analysis of normal human lymphoid tissues indicated that BCL-6 expression is topographically restricted to germinal centers including all centroblasts and centrocytes. The BCL-6 protein was also detectable in inter- and intrafollicular CD4+ T cells, but not in other follicular components including mantle-zone B cells, plasma cells, dendritic cells, and macrophages. Immunohistochemical analysis of DLCL and FL biopsy samples showed that the BCL-6 protein is detectable in these tumors independent of the presence of BCL-6 gene rearrangements. These results indicate that the expression of the BCL-6 gene is specifically regulated during B-cell differentiation and suggest a role for BCL-6 in germinal center development or function. Because DLCL derive from germinal-center B cells, deregulated BCL-6 expression may contribute to lymphomagenesis by preventing postgerminal center differentiation.",
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AU - Cattoretti, G.

AU - Chang, C. C.

AU - Cechova, K.

AU - Zhang, J.

AU - Ye, B. Hilda

AU - Falini, B.

AU - Louie, D. C.

AU - Offit, K.

AU - Chaganti, R. S K

AU - Dalla-Favera, R.

PY - 1995

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N2 - Structural alterations of the 5' noncoding region of the BCL-6 gene have been found in 40% of diffuse large cell lymphoma (DLCL) and 5% to 10% of follicular lymphomas (FL), suggesting that deregulated BCL-6 expression may play a role in lymphomagenesis. Nucleotide sequencing of BCL-6 cDNA predicted a protein containing six zinc-finger domains, suggesting that it may function as a transcription factor. Using antisera raised against N- and C-terminal BCL-6 synthetic oligopeptides in immunoprecipitation, immunoblot, and immunocytochemical assays, this study identifies the BCL-6 gene product as a 95-kD nuclear protein. Western blot analysis of human tumor cell lines representative of various hematopoietic lineages/stages of differentiation showed that the BCL-6 protein is predominantly expressed in the B-cell lineage where it was found in mature B cells. Immunohistochemical analysis of normal human lymphoid tissues indicated that BCL-6 expression is topographically restricted to germinal centers including all centroblasts and centrocytes. The BCL-6 protein was also detectable in inter- and intrafollicular CD4+ T cells, but not in other follicular components including mantle-zone B cells, plasma cells, dendritic cells, and macrophages. Immunohistochemical analysis of DLCL and FL biopsy samples showed that the BCL-6 protein is detectable in these tumors independent of the presence of BCL-6 gene rearrangements. These results indicate that the expression of the BCL-6 gene is specifically regulated during B-cell differentiation and suggest a role for BCL-6 in germinal center development or function. Because DLCL derive from germinal-center B cells, deregulated BCL-6 expression may contribute to lymphomagenesis by preventing postgerminal center differentiation.

AB - Structural alterations of the 5' noncoding region of the BCL-6 gene have been found in 40% of diffuse large cell lymphoma (DLCL) and 5% to 10% of follicular lymphomas (FL), suggesting that deregulated BCL-6 expression may play a role in lymphomagenesis. Nucleotide sequencing of BCL-6 cDNA predicted a protein containing six zinc-finger domains, suggesting that it may function as a transcription factor. Using antisera raised against N- and C-terminal BCL-6 synthetic oligopeptides in immunoprecipitation, immunoblot, and immunocytochemical assays, this study identifies the BCL-6 gene product as a 95-kD nuclear protein. Western blot analysis of human tumor cell lines representative of various hematopoietic lineages/stages of differentiation showed that the BCL-6 protein is predominantly expressed in the B-cell lineage where it was found in mature B cells. Immunohistochemical analysis of normal human lymphoid tissues indicated that BCL-6 expression is topographically restricted to germinal centers including all centroblasts and centrocytes. The BCL-6 protein was also detectable in inter- and intrafollicular CD4+ T cells, but not in other follicular components including mantle-zone B cells, plasma cells, dendritic cells, and macrophages. Immunohistochemical analysis of DLCL and FL biopsy samples showed that the BCL-6 protein is detectable in these tumors independent of the presence of BCL-6 gene rearrangements. These results indicate that the expression of the BCL-6 gene is specifically regulated during B-cell differentiation and suggest a role for BCL-6 in germinal center development or function. Because DLCL derive from germinal-center B cells, deregulated BCL-6 expression may contribute to lymphomagenesis by preventing postgerminal center differentiation.

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