@inbook{d5ff48c2bfd940d9924ec5b4b248d0ed,
title = "BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy",
abstract = "Biomolecular nuclear magnetic resonance (NMR) is a powerful and versatile method for studying both protein–protein interactions (PPIs) and protein–small molecule binding. NMR has been used extensively in the investigation of BCL-2 family proteins revealing the structure of key family members, identifying binding partners and interaction sites, and screening small molecule modulators. In this chapter we discuss the application of NMR to identify interaction sites and structure determination of protein–protein and protein–small molecule complexes using two examples.",
keywords = "BAX, BCL-2, BCL-2 family, Chemical shift perturbation (CSP), MCL-1, Nuclear magnetic resonance (NMR), Paramagnetic relaxation enhancement (PRE), Protein–protein interactions (PPI)",
author = "Garner, {Thomas P.} and Evripidis Gavathiotis",
note = "Funding Information: We would like to thank current and past members of the Gavathiotis Laboratory for contributing to the optimization of this protocol and research. This work was supported by an NCI grant 1R01CA178394 and awards from the Sidney Kimmel Foundation for Cancer Research, the Gabrielle{\textquoteright}s Angels Foundation for Cancer Research, the Alexandrine and Alexander L. Sinsheimer Foundation, the Pershing Square Sohn Cancer Research Alliance, the American Heart Association Collaborative Science Award (15CSA26240000), the Fondation Leducq Transatlantic Network of Excellence grant (RA15CVD04) and the Irma T. Hirschl Trust Career Award. NMR data were collected with support from NIH awards 1S10OD016305 and P30 CA013330. Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2019",
doi = "10.1007/978-1-4939-8861-7_15",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "217--231",
booktitle = "Methods in Molecular Biology",
}