BCL-2 expression or antioxidants prevent hyperglycemia-induced formation of intracellular advanced glycation endproducts in bovine endothelial cells

Ida Giardino, Diane Edelstein, Michael Brownlee

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

Hyperglycemia rapidly induces an increase in intracellular advanced glycation end products (AGEs) in bovine endothelial cells, causing an alteration in bFGF activity (Giardino, I., D. Edelstein, and M. Brownlee. 1994. J. Clin. Invest. 94:110-117). Because sugar or sugar-adduct autoxidation is critical for AGE formation in vitro, we evaluated the role of reactive oxygen species (ROS) in intracellular AGE formation, using bovine aortic endothelial cells. 30 mM glucose increased intracellular ROS formation by 250% and lipid peroxidation by 330%, while not affecting ROS in the media. In cells depleted of glutathione, intracellular AGE accumulation increased linearly with ROS generation as measured by immunoblotting and the fluorescent probe DCFH (AGE 0.258-3.531 AU* mm/5 x 104 cells, DCF 57-149 mean AU, r = .998, P < .002). Deferoxamine, α-tocopherol, and dimethylsulfoxide each inhibited hyperglycemia-induced formation of both ROS and AGE. To differentiate an effect of ROS generation on AGE formation from an effect of more distal oxidative processes, GM7373 endothelial cell lines were generated that stably expressed the peroxidation-suppressing proto- oncogene bcl-2. bcl-2 had no effect on hyperglycemia-induced intracellular ROS formation. In contrast, bcl-2 expression decreased both lipid peroxidation (100% at 3 h and 29% at 168 h) and AGE formation (55% at 168 h). These data show that a ROS-dependent process plays a central role in the generation of intracellular AGEs, and that inhibition of oxidant pathways prevents intracellular AGE formation.

Original languageEnglish (US)
Pages (from-to)1422-1428
Number of pages7
JournalJournal of Clinical Investigation
Volume97
Issue number6
StatePublished - Mar 15 1996

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Hyperglycemia
Reactive Oxygen Species
Endothelial Cells
Antioxidants
Lipid Peroxidation
Advanced Glycosylation End Products
Deferoxamine
Tocopherols
Proto-Oncogenes
Dimethyl Sulfoxide
Fluorescent Dyes
Immunoblotting
Oxidants
Glutathione
Glucose
Cell Line

Keywords

  • antioxidants
  • diabetes
  • glucose
  • peroxidation
  • reactive oxygen species

ASJC Scopus subject areas

  • Medicine(all)

Cite this

BCL-2 expression or antioxidants prevent hyperglycemia-induced formation of intracellular advanced glycation endproducts in bovine endothelial cells. / Giardino, Ida; Edelstein, Diane; Brownlee, Michael.

In: Journal of Clinical Investigation, Vol. 97, No. 6, 15.03.1996, p. 1422-1428.

Research output: Contribution to journalArticle

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