TY - JOUR
T1 - Banff 2019 Meeting Report
T2 - Molecular diagnostics in solid organ transplantation–Consensus for the Banff Human Organ Transplant (B-HOT) gene panel and open source multicenter validation
AU - Mengel, Michael
AU - Loupy, Alexandre
AU - Haas, Mark
AU - Roufosse, Candice
AU - Naesens, Maarten
AU - Akalin, Enver
AU - Clahsen-van Groningen, Marian C.
AU - Dagobert, Jessy
AU - Demetris, Anthony J.
AU - Duong van Huyen, Jean Paul
AU - Gueguen, Juliette
AU - Issa, Fadi
AU - Robin, Blaise
AU - Rosales, Ivy
AU - Von der Thüsen, Jan H.
AU - Sanchez-Fueyo, Alberto
AU - Smith, Rex N.
AU - Wood, Kathryn
AU - Adam, Benjamin
AU - Colvin, Robert B.
N1 - Publisher Copyright:
© 2020 The Authors. American Journal of Transplantation published by Wiley Periodicals LLC on behalf of The American Society of Transplantation and the American Society of Transplant Surgeons
PY - 2020/9/1
Y1 - 2020/9/1
N2 - This meeting report from the XV Banff conference describes the creation of a multiorgan transplant gene panel by the Banff Molecular Diagnostics Working Group (MDWG). This Banff Human Organ Transplant (B-HOT) panel is the culmination of previous work by the MDWG to identify a broadly useful gene panel based on whole transcriptome technology. A data-driven process distilled a gene list from peer-reviewed comprehensive microarray studies that discovered and validated their use in kidney, liver, heart, and lung transplant biopsies. These were supplemented by genes that define relevant cellular pathways and cell types plus 12 reference genes used for normalization. The 770 gene B-HOT panel includes the most pertinent genes related to rejection, tolerance, viral infections, and innate and adaptive immune responses. This commercially available panel uses the NanoString platform, which can quantitate transcripts from formalin-fixed paraffin-embedded samples. The B-HOT panel will facilitate multicenter collaborative clinical research using archival samples and permit the development of an open source large database of standardized analyses, thereby expediting clinical validation studies. The MDWG believes that a pathogenesis and pathway based molecular approach will be valuable for investigators and promote therapeutic decision-making and clinical trials.
AB - This meeting report from the XV Banff conference describes the creation of a multiorgan transplant gene panel by the Banff Molecular Diagnostics Working Group (MDWG). This Banff Human Organ Transplant (B-HOT) panel is the culmination of previous work by the MDWG to identify a broadly useful gene panel based on whole transcriptome technology. A data-driven process distilled a gene list from peer-reviewed comprehensive microarray studies that discovered and validated their use in kidney, liver, heart, and lung transplant biopsies. These were supplemented by genes that define relevant cellular pathways and cell types plus 12 reference genes used for normalization. The 770 gene B-HOT panel includes the most pertinent genes related to rejection, tolerance, viral infections, and innate and adaptive immune responses. This commercially available panel uses the NanoString platform, which can quantitate transcripts from formalin-fixed paraffin-embedded samples. The B-HOT panel will facilitate multicenter collaborative clinical research using archival samples and permit the development of an open source large database of standardized analyses, thereby expediting clinical validation studies. The MDWG believes that a pathogenesis and pathway based molecular approach will be valuable for investigators and promote therapeutic decision-making and clinical trials.
KW - biomarker
KW - biopsy
KW - classification systems: Banff classification
KW - clinical research/practice
KW - diagnostic techniques and imaging
KW - pathology/histopathology
UR - http://www.scopus.com/inward/record.url?scp=85087158753&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087158753&partnerID=8YFLogxK
U2 - 10.1111/ajt.16059
DO - 10.1111/ajt.16059
M3 - Article
C2 - 32428337
AN - SCOPUS:85087158753
SN - 1600-6135
VL - 20
SP - 2305
EP - 2317
JO - American Journal of Transplantation
JF - American Journal of Transplantation
IS - 9
ER -