Bacterial phagosome acidification within IFN-γ-Activated macrophages

Role of host p47 immunity-related GTPases IRGs)

Sangeeta Tiwari, John D. MacMicking

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Interferon-gamma IFN-γ)-induced remodeling of the bacterial phagosome for pathogen clearance elicits the aid of a new family of GTPases termed the p47 IRGs. Members of this group reside primarily on ER-Golgi membranes before translocating to the nascent phagosome within minutes of bacterial uptake. Recruitment of p47 IRGs coincides with the acquisition of phagosome maturation and autophagy markers as well as enhanced acidification of this organelle. Here we describe a simple spectrofluorometric assay to measure luminal acidification of the bacterial phagosome within intact cells such as macrophages. This method can be applied to study the phagosomal pH pH-pg) of activated cells infected with a variety of infectious microorganisms and the roles played by members of the p47 IRG family in auto)phagolysosome biogenesis.

Original languageEnglish (US)
Title of host publicationAutophagosome and Phagosome
PublisherHumana Press
Pages407-415
Number of pages9
Volume445
ISBN (Print)9781588298539
DOIs
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume445
ISSN (Print)10643745

Fingerprint

Phagosomes
GTP Phosphohydrolases
Immunity
Macrophages
Autophagy
Organelles
Interferon-gamma
Membranes

Keywords

  • Bacterial phagosome
  • IFN-γ
  • Macrophage
  • P47 immunity-related GTPase
  • Phagosome acidification.

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Tiwari, S., & MacMicking, J. D. (2008). Bacterial phagosome acidification within IFN-γ-Activated macrophages: Role of host p47 immunity-related GTPases IRGs). In Autophagosome and Phagosome (Vol. 445, pp. 407-415). (Methods in Molecular Biology; Vol. 445). Humana Press. https://doi.org/10.1007/978-1-59745-157-4_26

Bacterial phagosome acidification within IFN-γ-Activated macrophages : Role of host p47 immunity-related GTPases IRGs). / Tiwari, Sangeeta; MacMicking, John D.

Autophagosome and Phagosome. Vol. 445 Humana Press, 2008. p. 407-415 (Methods in Molecular Biology; Vol. 445).

Research output: Chapter in Book/Report/Conference proceedingChapter

Tiwari, S & MacMicking, JD 2008, Bacterial phagosome acidification within IFN-γ-Activated macrophages: Role of host p47 immunity-related GTPases IRGs). in Autophagosome and Phagosome. vol. 445, Methods in Molecular Biology, vol. 445, Humana Press, pp. 407-415. https://doi.org/10.1007/978-1-59745-157-4_26
Tiwari S, MacMicking JD. Bacterial phagosome acidification within IFN-γ-Activated macrophages: Role of host p47 immunity-related GTPases IRGs). In Autophagosome and Phagosome. Vol. 445. Humana Press. 2008. p. 407-415. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-157-4_26
Tiwari, Sangeeta ; MacMicking, John D. / Bacterial phagosome acidification within IFN-γ-Activated macrophages : Role of host p47 immunity-related GTPases IRGs). Autophagosome and Phagosome. Vol. 445 Humana Press, 2008. pp. 407-415 (Methods in Molecular Biology).
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