Bacterial phagosome acidification within IFN-γ-Activated macrophages: Role of host p47 immunity-related GTPases IRGs)

Sangeeta Tiwari, John D. MacMicking

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Interferon-gamma IFN-γ)-induced remodeling of the bacterial phagosome for pathogen clearance elicits the aid of a new family of GTPases termed the p47 IRGs. Members of this group reside primarily on ER-Golgi membranes before translocating to the nascent phagosome within minutes of bacterial uptake. Recruitment of p47 IRGs coincides with the acquisition of phagosome maturation and autophagy markers as well as enhanced acidification of this organelle. Here we describe a simple spectrofluorometric assay to measure luminal acidification of the bacterial phagosome within intact cells such as macrophages. This method can be applied to study the phagosomal pH pH-pg) of activated cells infected with a variety of infectious microorganisms and the roles played by members of the p47 IRG family in auto)phagolysosome biogenesis.

Original languageEnglish (US)
Title of host publicationAutophagosome and Phagosome
PublisherHumana Press
Pages407-415
Number of pages9
ISBN (Print)9781588298539
DOIs
Publication statusPublished - Jan 1 2008

Publication series

NameMethods in Molecular Biology
Volume445
ISSN (Print)1064-3745

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Keywords

  • Bacterial phagosome
  • IFN-γ
  • Macrophage
  • P47 immunity-related GTPase
  • Phagosome acidification.

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Tiwari, S., & MacMicking, J. D. (2008). Bacterial phagosome acidification within IFN-γ-Activated macrophages: Role of host p47 immunity-related GTPases IRGs). In Autophagosome and Phagosome (pp. 407-415). (Methods in Molecular Biology; Vol. 445). Humana Press. https://doi.org/10.1007/978-1-59745-157-4_26