@article{67a05474fece48ee9581f679334fb6da,
title = "Background free imaging of single mRNAs in live cells using split fluorescent proteins",
abstract = "We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.",
author = "Bin Wu and Jiahao Chen and Singer, {Robert H.}",
note = "Funding Information: This work was supported by the grant GM84364 and EB 013571 from National Institutes of Health to R.H.S. The plasmid eqFP670 was a kind gift from Dr. Vladislav Verkusha, Albert Einstein College of Medicine. The authors thank Xiuhua Meng and Jefferey A. Chao for cloning.",
year = "2014",
month = jan,
day = "9",
doi = "10.1038/srep03615",
language = "English (US)",
volume = "4",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}