Background free imaging of single mRNAs in live cells using split fluorescent proteins

Bin Wu, Jiahao Chen, Robert H. Singer

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.

Original languageEnglish (US)
Article number3615
JournalScientific Reports
Volume4
DOIs
StatePublished - Jan 9 2014

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Levivirus
Messenger RNA
Capsid Proteins
Proteins
Fluorescence
RNA
RNA-Binding Motifs

ASJC Scopus subject areas

  • General

Cite this

Background free imaging of single mRNAs in live cells using split fluorescent proteins. / Wu, Bin; Chen, Jiahao; Singer, Robert H.

In: Scientific Reports, Vol. 4, 3615, 09.01.2014.

Research output: Contribution to journalArticle

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