Bacillus calmette-guerin-activated murine macrophages kill syngeneic melanoma cells under strict anaerobic conditions

Victoria H. Freedman, T. E. Gorrell, C. F. Nathan

Research output: Contribution to journalArticle

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Abstract

We have studied the spontaneous killing of B559 melanoma cells by Bacillus Calmette-Guerin (BCG)-elicited macrophages under strictly anaerobic conditions to investigate the role of oxygen in macrophage-mediated cytotoxicity. The number of melanoma cells capable of forming colonies after aerobic or anaerobic incubation with BCG-macrophages was used as the index of cytotoxicity. The BCG-macrophages killed melanoma cells regardless of the amount of oxygen present. The killing observed was proportional to the ratio of effector cells added; a ratio of 25:1 effector to target cells was required to achieve nearly 90% cytotoxicity both aerobically and anaerobically. This cytotoxicity was not dependent on a diffusible macrophage product nor on alteration of the medium by macrophages, since tumor cells incubated in the same culture medium, but not in contact with a mixed population of tumor cells and macrophages, were not killed. These results also indicated that macrophage-mediated cytotoxicity was dependent on macrophage-tumor cell contact. The mechanism responsible for the oxygen-independent cytotoxicity is unknown at present.

Original languageEnglish (US)
Pages (from-to)94-97
Number of pages4
JournalJournal of Experimental Medicine
Volume160
Issue number1
StatePublished - 1984
Externally publishedYes

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Mycobacterium bovis
Melanoma
Macrophages
Oxygen
Neoplasms
Culture Media
Cell Count

ASJC Scopus subject areas

  • Immunology

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Bacillus calmette-guerin-activated murine macrophages kill syngeneic melanoma cells under strict anaerobic conditions. / Freedman, Victoria H.; Gorrell, T. E.; Nathan, C. F.

In: Journal of Experimental Medicine, Vol. 160, No. 1, 1984, p. 94-97.

Research output: Contribution to journalArticle

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