TY - JOUR
T1 - Role of B lymphocytes in cell-mediated immunity
T2 - I. requirement for T cells or T-cell products for antigen-induced B-cell activation
AU - Wahl, S. M.
AU - Rosenstreich, D. L.
PY - 1976/11/2
Y1 - 1976/11/2
N2 - Although B lymphocytes can be triggered by B-cell mitogens and by certain other molecules to produce tymphokines, they do not produce lymphokines when stimulated with specific soluble protein antigens. We have investigated whether T-cell help would enable B cells to produce lymphokines when activated by antigens. Addition of small numbers of T cells to B-cell cultures resulted in significant production of a monocyte chemotactic factor. T cells could be replaced by supernates of antigen-stimulated T cells, demonstrating both that the chemotactic factor was B-cell-derived and that T-cell help was mediated by a soluble factor. Although the T-cell factor was nonantigen specific, B-cell activation required the presence of both antigen and T-cell factor. Thus, it appears that although dependent upon T cells, B lymphocytes may play an important role in amplification of cell-mediated immune responses.
AB - Although B lymphocytes can be triggered by B-cell mitogens and by certain other molecules to produce tymphokines, they do not produce lymphokines when stimulated with specific soluble protein antigens. We have investigated whether T-cell help would enable B cells to produce lymphokines when activated by antigens. Addition of small numbers of T cells to B-cell cultures resulted in significant production of a monocyte chemotactic factor. T cells could be replaced by supernates of antigen-stimulated T cells, demonstrating both that the chemotactic factor was B-cell-derived and that T-cell help was mediated by a soluble factor. Although the T-cell factor was nonantigen specific, B-cell activation required the presence of both antigen and T-cell factor. Thus, it appears that although dependent upon T cells, B lymphocytes may play an important role in amplification of cell-mediated immune responses.
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U2 - 10.1084/jem.144.5.1175
DO - 10.1084/jem.144.5.1175
M3 - Article
C2 - 1086881
AN - SCOPUS:0017102098
SN - 0022-1007
VL - 144
SP - 1175
EP - 1187
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 5
ER -