B-LINK: A Hemicentin, Plakin, and Integrin-Dependent Adhesion System that Links Tissues by Connecting Adjacent Basement Membranes

Meghan A. Morrissey, Daniel P. Keeley, Elliott J. Hagedorn, Shelly T H McClatchey, Qiuyi Chi, David H. Hall, David R. Sherwood

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Basement membrane (BM), a sheet-like form of extracellular matrix, surrounds most tissues. Duringorganogenesis, specific adhesions between adjoining tissues frequently occur; however, their molecular basis is unclear. Using live-cell imaging and electron microscopy, we identify an adhesion system that connects the uterine and gonadal tissues through their juxtaposed BMs at the site of anchor cell (AC) invasion in C.elegans. We find that the extracellular matrix component hemicentin (HIM-4), found between BMs, forms punctate accumulations under the AC and controls BM linkage to promote rapid invasion. Through targeted screening, we identify the integrin-binding cytolinker plakin (VAB-10A) and integrin (INA-1/PAT-3) as key BM-BM linkage regulators: VAB-10A localizes to the AC-BM interface and tethers hemicentin to the AC while integrin promotes hemicentin punctae formation. Together, plakin, integrin, and hemicentin are founding components of a cell-directed adhesion system, which we name a BM-. LINKage (B-LINK), that connects adjacent tissues through adjoining BMs.

Original languageEnglish (US)
Pages (from-to)319-331
Number of pages13
JournalDevelopmental Cell
Volume31
Issue number3
DOIs
StatePublished - Nov 10 2014

Fingerprint

Basement Membrane
Integrins
Adhesion
Tissue
Anchors
Extracellular Matrix
Cell Adhesion
Electron microscopy
Names
Electron Microscopy
Screening
Cell Membrane
Imaging techniques

ASJC Scopus subject areas

  • Developmental Biology

Cite this

B-LINK : A Hemicentin, Plakin, and Integrin-Dependent Adhesion System that Links Tissues by Connecting Adjacent Basement Membranes. / Morrissey, Meghan A.; Keeley, Daniel P.; Hagedorn, Elliott J.; McClatchey, Shelly T H; Chi, Qiuyi; Hall, David H.; Sherwood, David R.

In: Developmental Cell, Vol. 31, No. 3, 10.11.2014, p. 319-331.

Research output: Contribution to journalArticle

Morrissey, Meghan A. ; Keeley, Daniel P. ; Hagedorn, Elliott J. ; McClatchey, Shelly T H ; Chi, Qiuyi ; Hall, David H. ; Sherwood, David R. / B-LINK : A Hemicentin, Plakin, and Integrin-Dependent Adhesion System that Links Tissues by Connecting Adjacent Basement Membranes. In: Developmental Cell. 2014 ; Vol. 31, No. 3. pp. 319-331.
@article{d5e1176930a24a2c83461d11e10bc66b,
title = "B-LINK: A Hemicentin, Plakin, and Integrin-Dependent Adhesion System that Links Tissues by Connecting Adjacent Basement Membranes",
abstract = "Basement membrane (BM), a sheet-like form of extracellular matrix, surrounds most tissues. Duringorganogenesis, specific adhesions between adjoining tissues frequently occur; however, their molecular basis is unclear. Using live-cell imaging and electron microscopy, we identify an adhesion system that connects the uterine and gonadal tissues through their juxtaposed BMs at the site of anchor cell (AC) invasion in C.elegans. We find that the extracellular matrix component hemicentin (HIM-4), found between BMs, forms punctate accumulations under the AC and controls BM linkage to promote rapid invasion. Through targeted screening, we identify the integrin-binding cytolinker plakin (VAB-10A) and integrin (INA-1/PAT-3) as key BM-BM linkage regulators: VAB-10A localizes to the AC-BM interface and tethers hemicentin to the AC while integrin promotes hemicentin punctae formation. Together, plakin, integrin, and hemicentin are founding components of a cell-directed adhesion system, which we name a BM-. LINKage (B-LINK), that connects adjacent tissues through adjoining BMs.",
author = "Morrissey, {Meghan A.} and Keeley, {Daniel P.} and Hagedorn, {Elliott J.} and McClatchey, {Shelly T H} and Qiuyi Chi and Hall, {David H.} and Sherwood, {David R.}",
year = "2014",
month = "11",
day = "10",
doi = "10.1016/j.devcel.2014.08.024",
language = "English (US)",
volume = "31",
pages = "319--331",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - B-LINK

T2 - A Hemicentin, Plakin, and Integrin-Dependent Adhesion System that Links Tissues by Connecting Adjacent Basement Membranes

AU - Morrissey, Meghan A.

AU - Keeley, Daniel P.

AU - Hagedorn, Elliott J.

AU - McClatchey, Shelly T H

AU - Chi, Qiuyi

AU - Hall, David H.

AU - Sherwood, David R.

PY - 2014/11/10

Y1 - 2014/11/10

N2 - Basement membrane (BM), a sheet-like form of extracellular matrix, surrounds most tissues. Duringorganogenesis, specific adhesions between adjoining tissues frequently occur; however, their molecular basis is unclear. Using live-cell imaging and electron microscopy, we identify an adhesion system that connects the uterine and gonadal tissues through their juxtaposed BMs at the site of anchor cell (AC) invasion in C.elegans. We find that the extracellular matrix component hemicentin (HIM-4), found between BMs, forms punctate accumulations under the AC and controls BM linkage to promote rapid invasion. Through targeted screening, we identify the integrin-binding cytolinker plakin (VAB-10A) and integrin (INA-1/PAT-3) as key BM-BM linkage regulators: VAB-10A localizes to the AC-BM interface and tethers hemicentin to the AC while integrin promotes hemicentin punctae formation. Together, plakin, integrin, and hemicentin are founding components of a cell-directed adhesion system, which we name a BM-. LINKage (B-LINK), that connects adjacent tissues through adjoining BMs.

AB - Basement membrane (BM), a sheet-like form of extracellular matrix, surrounds most tissues. Duringorganogenesis, specific adhesions between adjoining tissues frequently occur; however, their molecular basis is unclear. Using live-cell imaging and electron microscopy, we identify an adhesion system that connects the uterine and gonadal tissues through their juxtaposed BMs at the site of anchor cell (AC) invasion in C.elegans. We find that the extracellular matrix component hemicentin (HIM-4), found between BMs, forms punctate accumulations under the AC and controls BM linkage to promote rapid invasion. Through targeted screening, we identify the integrin-binding cytolinker plakin (VAB-10A) and integrin (INA-1/PAT-3) as key BM-BM linkage regulators: VAB-10A localizes to the AC-BM interface and tethers hemicentin to the AC while integrin promotes hemicentin punctae formation. Together, plakin, integrin, and hemicentin are founding components of a cell-directed adhesion system, which we name a BM-. LINKage (B-LINK), that connects adjacent tissues through adjoining BMs.

UR - http://www.scopus.com/inward/record.url?scp=84922743665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922743665&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2014.08.024

DO - 10.1016/j.devcel.2014.08.024

M3 - Article

C2 - 25443298

AN - SCOPUS:84922743665

VL - 31

SP - 319

EP - 331

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 3

ER -