TY - JOUR
T1 - B-LINK
T2 - A Hemicentin, Plakin, and Integrin-Dependent Adhesion System that Links Tissues by Connecting Adjacent Basement Membranes
AU - Morrissey, Meghan A.
AU - Keeley, Daniel P.
AU - Hagedorn, Elliott J.
AU - McClatchey, Shelly T.H.
AU - Chi, Qiuyi
AU - Hall, David H.
AU - Sherwood, David R.
N1 - Funding Information:
We thank N. Medvitz and S. Miller at Duke Electron Microscopy Services; K. Nguyen at the Center for C. elegans Anatomy; B. Vogel and M. Labouesse for strains and discussions, and L. Kelley, L. Lohmer, D. Matus, and J. Pelton for comments on the manuscript. Some strains were provided by the Caenorhabditis Genetics Center, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). The TEM image shown in Figure S1 comes from the laboratory of Sydney Brenner at MRC/LMB in Cambridge England, provided courtesy of John White and Jonathan Hodgkin. Curation of the Brenner EM archive in David Hall’s laboratory in New York is supported by NIH grant OD010943 to D.H.H. This work was supported by The Pew Scholars Program in the Biomedical Sciences and NIH Grants GM079320 and GM100083 to D.R.S. and a National Science Foundation Graduate Research Fellowship to M.A.M.
Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/11/10
Y1 - 2014/11/10
N2 - Basement membrane (BM), a sheet-like form of extracellular matrix, surrounds most tissues. Duringorganogenesis, specific adhesions between adjoining tissues frequently occur; however, their molecular basis is unclear. Using live-cell imaging and electron microscopy, we identify an adhesion system that connects the uterine and gonadal tissues through their juxtaposed BMs at the site of anchor cell (AC) invasion in C.elegans. We find that the extracellular matrix component hemicentin (HIM-4), found between BMs, forms punctate accumulations under the AC and controls BM linkage to promote rapid invasion. Through targeted screening, we identify the integrin-binding cytolinker plakin (VAB-10A) and integrin (INA-1/PAT-3) as key BM-BM linkage regulators: VAB-10A localizes to the AC-BM interface and tethers hemicentin to the AC while integrin promotes hemicentin punctae formation. Together, plakin, integrin, and hemicentin are founding components of a cell-directed adhesion system, which we name a BM-. LINKage (B-LINK), that connects adjacent tissues through adjoining BMs.
AB - Basement membrane (BM), a sheet-like form of extracellular matrix, surrounds most tissues. Duringorganogenesis, specific adhesions between adjoining tissues frequently occur; however, their molecular basis is unclear. Using live-cell imaging and electron microscopy, we identify an adhesion system that connects the uterine and gonadal tissues through their juxtaposed BMs at the site of anchor cell (AC) invasion in C.elegans. We find that the extracellular matrix component hemicentin (HIM-4), found between BMs, forms punctate accumulations under the AC and controls BM linkage to promote rapid invasion. Through targeted screening, we identify the integrin-binding cytolinker plakin (VAB-10A) and integrin (INA-1/PAT-3) as key BM-BM linkage regulators: VAB-10A localizes to the AC-BM interface and tethers hemicentin to the AC while integrin promotes hemicentin punctae formation. Together, plakin, integrin, and hemicentin are founding components of a cell-directed adhesion system, which we name a BM-. LINKage (B-LINK), that connects adjacent tissues through adjoining BMs.
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U2 - 10.1016/j.devcel.2014.08.024
DO - 10.1016/j.devcel.2014.08.024
M3 - Article
C2 - 25443298
AN - SCOPUS:84922743665
SN - 1534-5807
VL - 31
SP - 319
EP - 331
JO - Developmental Cell
JF - Developmental Cell
IS - 3
ER -