B and T cell epitope mapping and study the humoral and cell mediated immune response to B-T constructs of YscF antigen of Yersinia pestis

Riyasat Ali, Sudhir Kumar, Raza Ali Naqvi, D. N. Rao

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

YscF antigen, a type III secretion protein has recently been shown partial protection in murine model. Five peptides of YscF antigen were predicted using DNASTAR and T-cell prediction software. Peptides were synthesised and authenticated using competitive, direct binding immunoassay with anti YscF/peptide sera raised in mice. Peptide P1 and P2 were found to be B cell epitope while P3 was minor B cell epitope. P4 peptide was a pure T cell epitope based on lymphoproliferative response, cytokines profile and T-bet expression. Furthermore, with an intention to enhance immunogenicity, three B-T constructs were designed between the above epitopes. Conjugate B1T1 and B2T1 showed higher serum IgG/IgA titre, respectively, as well as high secretory IgA plus secretory component (Sc) both in lung and intestinal washes. Also, these conjugates showed high T-cell proliferation in addition to higher Th1 type cytokines (IFN-γ and IL-2) in cells obtained from spleen, lamina propria and Peyer's patches. B3T1 stimulated cells showed moderate levels of IFN-γ and IL-2 but higher levels of IL-4. This study demonstrates superior immunogen of B1T1 and B2T1 of YscF antigen to be exploited as vaccine candidate for plague.

Original languageEnglish (US)
Pages (from-to)365-378
Number of pages14
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume36
Issue number4
DOIs
StatePublished - Jul 1 2013
Externally publishedYes

Fingerprint

B-Lymphocyte Epitopes
Epitope Mapping
Yersinia pestis
T-Lymphocyte Epitopes
cell-mediated immunity
B-lymphocytes
T-lymphocytes
epitopes
peptides
antigens
Antigens
Peptides
Interleukin-2
Plague Vaccine
Cytokines
Secretory Component
T-Lymphocytes
Secretory Immunoglobulin A
Peyer's Patches
interleukin-2

Keywords

  • B-T construct
  • Cytokine
  • Epitope
  • Yersinia pestis
  • YscF antigen

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

Cite this

B and T cell epitope mapping and study the humoral and cell mediated immune response to B-T constructs of YscF antigen of Yersinia pestis. / Ali, Riyasat; Kumar, Sudhir; Naqvi, Raza Ali; Rao, D. N.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 36, No. 4, 01.07.2013, p. 365-378.

Research output: Contribution to journalArticle

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