Abstract
To determine the functional role for and the pharmacological specificity of developing gastrointestinal CCK receptors, in vitro pyloric contractility and autoradiographic CCK receptor binding were examined in pups aged 1-20 days. CCK contracted the gastroduodenal junction at all ages, while nonsulfated CCK-8 (d-CCK) was less potent. Autoradiographic studies revealed CCK binding localized to the gastroduodenal junction throughout development. MK-329, a specific type A CCK receptor antagonist, completely displaced 125I CCK-8 binding at all ages, while d-CCK displaced binding at ages at which d-CCK elicited gastroduodenal contractility. The results demonstrate a physiological role for and pharmacological specificity of neonatal gastroduodenal CCK receptors.
Original language | English (US) |
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Pages (from-to) | 1199-1203 |
Number of pages | 5 |
Journal | Peptides |
Volume | 11 |
Issue number | 6 |
DOIs | |
State | Published - 1990 |
Externally published | Yes |
Keywords
- Autoradiography
- CCK
- Gastric emptying
- Ingestive behavior
- Neonatal rat
- Ontogeny
- Pylorus
- Receptors
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Endocrinology
- Cellular and Molecular Neuroscience