Autophagy genes are essential for dauer development and life-span extension in C. elegans

Alicia Meléndez, Zsolt Tallóczy, Matthew Seaman, Eeva Liisa Eskelinen, David H. Hall, Beth Levine

Research output: Contribution to journalArticle

862 Citations (Scopus)

Abstract

Both dauer formation (a stage of developmental arrest) and adult life-span in Caenorhabditis elegans are negatively regulated by insulin-like signaling, but little is known about cellular pathways that mediate these processes. Autophagy, through the sequestration and delivery of cargo to the lysosomes, is the major route for degrading long-lived proteins and cytoplasmic organelles in eukaryotic cells. Using nematodes with a loss-of-function mutation in the insulin-like signaling pathway, we show that bec-1, the C. elegans ortholog of the yeast and mammalian autophagy gene APG6/VPS30/beclin1, is essential for normal dauer morphogenesis and life-span extension. Dauer formation is associated with increased autophagy and also requires C. elegans orthologs of the yeast autophagy genes APG1, APG7, APG8, and AUT10. Thus, autophagy is a cellular pathway essential for dauer development and life-span extension in C. elegans.

Original languageEnglish (US)
Pages (from-to)1387-1391
Number of pages5
JournalScience
Volume301
Issue number5638
DOIs
StatePublished - Sep 5 2003

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Essential Genes
Autophagy
Life Expectancy
Caenorhabditis elegans
Yeasts
Insulin
Eukaryotic Cells
Lysosomes
Morphogenesis
Organelles
Genes
Mutation
Proteins

ASJC Scopus subject areas

  • General

Cite this

Meléndez, A., Tallóczy, Z., Seaman, M., Eskelinen, E. L., Hall, D. H., & Levine, B. (2003). Autophagy genes are essential for dauer development and life-span extension in C. elegans. Science, 301(5638), 1387-1391. https://doi.org/10.1126/science.1087782

Autophagy genes are essential for dauer development and life-span extension in C. elegans. / Meléndez, Alicia; Tallóczy, Zsolt; Seaman, Matthew; Eskelinen, Eeva Liisa; Hall, David H.; Levine, Beth.

In: Science, Vol. 301, No. 5638, 05.09.2003, p. 1387-1391.

Research output: Contribution to journalArticle

Meléndez, A, Tallóczy, Z, Seaman, M, Eskelinen, EL, Hall, DH & Levine, B 2003, 'Autophagy genes are essential for dauer development and life-span extension in C. elegans', Science, vol. 301, no. 5638, pp. 1387-1391. https://doi.org/10.1126/science.1087782
Meléndez A, Tallóczy Z, Seaman M, Eskelinen EL, Hall DH, Levine B. Autophagy genes are essential for dauer development and life-span extension in C. elegans. Science. 2003 Sep 5;301(5638):1387-1391. https://doi.org/10.1126/science.1087782
Meléndez, Alicia ; Tallóczy, Zsolt ; Seaman, Matthew ; Eskelinen, Eeva Liisa ; Hall, David H. ; Levine, Beth. / Autophagy genes are essential for dauer development and life-span extension in C. elegans. In: Science. 2003 ; Vol. 301, No. 5638. pp. 1387-1391.
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