Autonomous screening of C. elegans identifies genes implicated in synaptogenesis

Matthew M. Crane, Jeffrey N. Stirman, Chan Yen Ou, Peri T. Kurshan, James M. Rehg, Kang Shen, Hang Lu

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Morphometric studies in multicellular organisms are generally performed manually because of the complexity of multidimensional features and lack of appropriate tools for handling these organisms. Here we present an integrated system that identifies and sorts Caenorhabditis elegans mutants with altered subcellular traits in real time without human intervention. We performed self-directed screens 100 times faster than manual screens and identified both genes and phenotypic classes involved in synapse formation.

Original languageEnglish (US)
Pages (from-to)977-980
Number of pages4
JournalNature Methods
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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