Autonomous screening of C. elegans identifies genes implicated in synaptogenesis

Matthew M. Crane; Jeffrey N. Stirman; Chan Yen Ou; Peri T. Kurshan; James M. Rehg; Kang Shen; Hang Lu

doi:10.1038/nmeth.2141

Autonomous screening of C. elegans identifies genes implicated in synaptogenesis

Matthew M. Crane, Jeffrey N. Stirman, Chan Yen Ou, Peri T. Kurshan, James M. Rehg, Kang Shen, Hang Lu

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

Morphometric studies in multicellular organisms are generally performed manually because of the complexity of multidimensional features and lack of appropriate tools for handling these organisms. Here we present an integrated system that identifies and sorts Caenorhabditis elegans mutants with altered subcellular traits in real time without human intervention. We performed self-directed screens 100 times faster than manual screens and identified both genes and phenotypic classes involved in synapse formation.

Original language	English (US)
Pages (from-to)	977-980
Number of pages	4
Journal	Nature Methods
Volume	9
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.2141
State	Published - Oct 2012
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.2141

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title = "Autonomous screening of C. elegans identifies genes implicated in synaptogenesis",

abstract = "Morphometric studies in multicellular organisms are generally performed manually because of the complexity of multidimensional features and lack of appropriate tools for handling these organisms. Here we present an integrated system that identifies and sorts Caenorhabditis elegans mutants with altered subcellular traits in real time without human intervention. We performed self-directed screens 100 times faster than manual screens and identified both genes and phenotypic classes involved in synapse formation.",

author = "Crane, {Matthew M.} and Stirman, {Jeffrey N.} and Ou, {Chan Yen} and Kurshan, {Peri T.} and Rehg, {James M.} and Kang Shen and Hang Lu",

note = "Funding Information: We thank funding from the US National Institutes of Health, US National Science Foundation, Alfred P. Sloan Foundation and Howard Hughes Medical Institute.",

year = "2012",

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AU - Crane, Matthew M.

AU - Stirman, Jeffrey N.

AU - Ou, Chan Yen

AU - Kurshan, Peri T.

AU - Rehg, James M.

AU - Shen, Kang

AU - Lu, Hang

N1 - Funding Information: We thank funding from the US National Institutes of Health, US National Science Foundation, Alfred P. Sloan Foundation and Howard Hughes Medical Institute.

PY - 2012/10

Y1 - 2012/10

N2 - Morphometric studies in multicellular organisms are generally performed manually because of the complexity of multidimensional features and lack of appropriate tools for handling these organisms. Here we present an integrated system that identifies and sorts Caenorhabditis elegans mutants with altered subcellular traits in real time without human intervention. We performed self-directed screens 100 times faster than manual screens and identified both genes and phenotypic classes involved in synapse formation.

AB - Morphometric studies in multicellular organisms are generally performed manually because of the complexity of multidimensional features and lack of appropriate tools for handling these organisms. Here we present an integrated system that identifies and sorts Caenorhabditis elegans mutants with altered subcellular traits in real time without human intervention. We performed self-directed screens 100 times faster than manual screens and identified both genes and phenotypic classes involved in synapse formation.

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Autonomous screening of C. elegans identifies genes implicated in synaptogenesis

Abstract

ASJC Scopus subject areas

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