TY - JOUR
T1 - Automated microfluidic compact disc (CD) cultivation system of Caenorhabditis elegans
AU - Kim, Nahui
AU - Dempsey, Catherine M.
AU - Zoval, Jim V.
AU - Sze, Ji Ying
AU - Madou, Marc J.
N1 - Funding Information:
The authors appreciate Horacio Kido of Rapid Analysis Systems for his technical help and the use of equipments of CNC-machined CD fabrication, and John Hines and Tony Ricco from NASA for their contribution in technical discussions. This work was supported by grants from the National Aeronautics and Space Administration (to M. Madou), NIHMH (to J. Sze) and Marie Curie Foundation (to C. Dempsey).
PY - 2007/3/26
Y1 - 2007/3/26
N2 - Microfabricated platforms for automated cultivation and behavioral observation of animals would be a valuable tool in physiological and genetic studies. We report an automated microfluidic compact disc (CD) system specialized for cultivating and monitoring Caenorhabditis elegans, proficient in automated feeding, waste removal and live-animal microscopy. The automated CD cultivation platform contains cultivation, nutrient, and waste chamber, channels connecting the chambers, and venting holes. The feeding and waste removal processes are achieved automatically using centrifugal force driven fluidics. Compared to other microfluidic technologies for moving small amounts of fluidic or suspended particles from site to site, this centrifuge-based system is well suited for functions such as flow sequencing, mixing, capillary measuring, and switching. Those functions can be implemented by exploiting centrifugal, coriolis and capillary forces combined with specific microfluidic network. Thus, the CD cultivating system has the capacity to incorporate diverse molecular, biochemical, and pharmacological assays for real-time physiological and behavioral tests of live-animals. This compact and fully automated microfabricated platform is designed to address the unique requirements of space biology due to the stringent limitations on power, size, weight, and crew intervention.
AB - Microfabricated platforms for automated cultivation and behavioral observation of animals would be a valuable tool in physiological and genetic studies. We report an automated microfluidic compact disc (CD) system specialized for cultivating and monitoring Caenorhabditis elegans, proficient in automated feeding, waste removal and live-animal microscopy. The automated CD cultivation platform contains cultivation, nutrient, and waste chamber, channels connecting the chambers, and venting holes. The feeding and waste removal processes are achieved automatically using centrifugal force driven fluidics. Compared to other microfluidic technologies for moving small amounts of fluidic or suspended particles from site to site, this centrifuge-based system is well suited for functions such as flow sequencing, mixing, capillary measuring, and switching. Those functions can be implemented by exploiting centrifugal, coriolis and capillary forces combined with specific microfluidic network. Thus, the CD cultivating system has the capacity to incorporate diverse molecular, biochemical, and pharmacological assays for real-time physiological and behavioral tests of live-animals. This compact and fully automated microfabricated platform is designed to address the unique requirements of space biology due to the stringent limitations on power, size, weight, and crew intervention.
KW - Animal assay
KW - Automated cultivation system
KW - C. elegans
KW - Microfluidics
UR - http://www.scopus.com/inward/record.url?scp=33947103636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33947103636&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2006.06.026
DO - 10.1016/j.snb.2006.06.026
M3 - Article
AN - SCOPUS:33947103636
SN - 0925-4005
VL - 122
SP - 511
EP - 518
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 2
ER -