Autoantibodies and autoantigens in the urine of SLE patients

Sandra A. Sciascia, Harold Dickensheets, Wendy Picking, Kristina Robson, Dong Wang, B. Hilda Ye, Liangjin Zhu, Dean A. Stetler

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Autoantibodies against RNA polymerase I (RNAPI), DNA, La and ribosomal P proteins were detected in the urine of systemic lupus erythematosus (SLE) patients, many with normal protein excretion rates. In a number of cases, the antibodies were detectable in the urine but not the serum sample of the same patient. The presence and relative concentrations of the urinary autoantibodies correlated with disease activity. RNAPI antigens were detected in the urine of SLE patients by radioimmunoassay and immunoblotting using rabbit antisera prepared against the purified holoenzyme. Immunoaffinity purification of the rabbit anti-RNAPI with SLE urine proteins resulted in antibodies directed primarily against the largest RNAPI subunit (S1; 194 kDa). Antibodies prepared against recombinant fusion proteins representing the DNA binding regions of human RNAPI(S1) reacted with a 35 kDa SLE urinary protein, a putative fragment of RNAPI(S1). Ribosomal protein P0 was detected in SLE patients' urine by immunoblotting, using rabbit antiserum prepared against recombinant human P1 fusion protein. The relative quantities of urinary P0 correlated with disease status. Analysis of urinary autoantibodies and corresponding antigens in conjunction with analysis of serum autoantibodies may be of value for the purpose of monitoring disease activity.

Original languageEnglish (US)
Pages (from-to)503-514
Number of pages12
JournalAutoimmunity
Volume37
Issue number6-7
DOIs
StatePublished - Sep 1 2004

Keywords

  • Anti-DNA
  • Autoantigens
  • RNA polymerase I
  • Ribosomal P proteins
  • SLE
  • Urinary autoantibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Sciascia, S. A., Dickensheets, H., Picking, W., Robson, K., Wang, D., Ye, B. H., Zhu, L., & Stetler, D. A. (2004). Autoantibodies and autoantigens in the urine of SLE patients. Autoimmunity, 37(6-7), 503-514. https://doi.org/10.1080/08916930410001713016