Augmented production of Panton-Valentine leukocidin toxin in methicillin-resistant and methicillin-susceptible Staphylococcus aureus Is associated with worse outcome in a murine skin infection model

Avanish K. Varshney, Luis R. Martinez, Stephanie M. Hamilton, Amy E. Bryant, Michael H. Levi, Philip Gialanella, Dennis L. Stevens, Bettina C. Fries

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The role of Panton-Valentine leukocidin (PVL) in Staphylococcus aureus infections is controversial. We used a mouse model of skin infection to compare the virulence of methicillin-resistant S. aureus (MRSA) and methicillin- susceptible S. aureus (MSSA) strains with different levels of PVL production. Differences in PVL production were not associated with mutations in the genes lukS-PV and lukF-PV. However, MSSA and MRSA strains that produced high levels of PVL caused larger skin abscesses, higher bacterial burdens, and more tissue inflammation than did low-PVL-producing strains. Together, these data suggest that (1) the effect of PVL on the pathogenesis of staphylococcal infection may depend on the level of toxin produced and (2) many strains of MSSA that cause soft-tissue infections produce higher levels of PVL than do MRSA strains.

Original languageEnglish (US)
Pages (from-to)92-96
Number of pages5
JournalJournal of Infectious Diseases
Volume201
Issue number1
DOIs
StatePublished - Jan 2010

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Methicillin Resistance
Methicillin
Staphylococcus aureus
Skin
Infection
Methicillin-Resistant Staphylococcus aureus
Staphylococcal Infections
Soft Tissue Infections
Panton-Valentine leukocidin
Abscess
Virulence
Inflammation
Mutation

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Augmented production of Panton-Valentine leukocidin toxin in methicillin-resistant and methicillin-susceptible Staphylococcus aureus Is associated with worse outcome in a murine skin infection model. / Varshney, Avanish K.; Martinez, Luis R.; Hamilton, Stephanie M.; Bryant, Amy E.; Levi, Michael H.; Gialanella, Philip; Stevens, Dennis L.; Fries, Bettina C.

In: Journal of Infectious Diseases, Vol. 201, No. 1, 01.2010, p. 92-96.

Research output: Contribution to journalArticle

Varshney, Avanish K. ; Martinez, Luis R. ; Hamilton, Stephanie M. ; Bryant, Amy E. ; Levi, Michael H. ; Gialanella, Philip ; Stevens, Dennis L. ; Fries, Bettina C. / Augmented production of Panton-Valentine leukocidin toxin in methicillin-resistant and methicillin-susceptible Staphylococcus aureus Is associated with worse outcome in a murine skin infection model. In: Journal of Infectious Diseases. 2010 ; Vol. 201, No. 1. pp. 92-96.
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