Attenuation of interleukin-1beta by pulsed electromagnetic fields after traumatic brain injury

Jonathan Rasouli, Rukmani Lekhraj, Nicholas M. White, Eugene S. Flamm, Arthur A. Pilla, Berish Strauch, Diana Casper

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Traumatic Brain Injury (TBI) is a major cause of morbidity and mortality in civilian and military populations. Interleukin-1beta (IL-1β) is a pro-inflammatory cytokine with a key role in the inflammatory response following TBI and studies indicate that attenuation of this cytokine improves behavioral outcomes. Pulsed electromagnetic fields (PEMF) can reduce inflammation after soft tissue injuries in animals and humans. Therefore, we explored whether PEMF signals could alter the course of IL-1β production in rats subjected to closed-head contusive weight-drop injuries (Marmarou method) and penetrating needle-stick brain injuries. Protein levels, measured by the Biorad assay, were not altered by injuries or PEMF treatment. In addition, we verified that IL-1β levels in cerebrospinal fluid (CSF) were proportional to injury severity in the contusion model. Results demonstrate that PEMF treatment attenuated IL-1β levels up to 10-fold in CSF within 6. h after contusive injury and also significantly suppressed IL-1β within 17-24. h after penetrating injury. In contrast, no differences in IL-1β were seen between PEMF-treated and control groups in brain homogenates. To the authors' knowledge, this is the first report of the use of PEMF to modulate an inflammatory cytokine after TBI. These results warrant further studies to assess the effects of PEMF on other inflammatory markers and functional outcomes.

Original languageEnglish (US)
Pages (from-to)4-8
Number of pages5
JournalNeuroscience Letters
Volume519
Issue number1
DOIs
StatePublished - Jun 21 2012

Fingerprint

Electromagnetic Fields
Interleukin-1beta
Wounds and Injuries
Cytokines
Cerebrospinal Fluid
Needlestick Injuries
Soft Tissue Injuries
Contusions
Traumatic Brain Injury
Brain Injuries
Head
Inflammation
Morbidity
Weights and Measures
Control Groups
Mortality
Brain
Population

Keywords

  • IL-1β
  • Inflammation
  • Interleukin-1beta
  • PEMF
  • Pulsed electromagnetic fields
  • TBI
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Attenuation of interleukin-1beta by pulsed electromagnetic fields after traumatic brain injury. / Rasouli, Jonathan; Lekhraj, Rukmani; White, Nicholas M.; Flamm, Eugene S.; Pilla, Arthur A.; Strauch, Berish; Casper, Diana.

In: Neuroscience Letters, Vol. 519, No. 1, 21.06.2012, p. 4-8.

Research output: Contribution to journalArticle

Rasouli, Jonathan ; Lekhraj, Rukmani ; White, Nicholas M. ; Flamm, Eugene S. ; Pilla, Arthur A. ; Strauch, Berish ; Casper, Diana. / Attenuation of interleukin-1beta by pulsed electromagnetic fields after traumatic brain injury. In: Neuroscience Letters. 2012 ; Vol. 519, No. 1. pp. 4-8.
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