Atherosclerotic aortic component quantification by noninvasive magnetic resonance imaging

An in vivo study in rabbits

Gérard Helft, Stephen G. Worthley, Valentin Fuster, Azfar G. Zaman, Clyde B. Schechter, Julio I. Osende, Oswaldo J. Rodriguez, Zahi A. Fayad, John T. Fallon, Juan J. Badimon

Research output: Contribution to journalArticle

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Abstract

OBJECTIVES: We sought to demonstrate the ability that noninvasive in vivo magnetic resonance imaging (MRI) has to quantify the different components within atherosclerotic plaque. BACKGROUND: Atherosclerotic plaque composition plays a critical role in both lesion stability and subsequent thrombogenicity. Noninvasive MRI is a promising tool for the characterization of plaque composition. METHODS: Thoracic and abdominal aortic atherosclerotic lesions were induced in rabbits (n = 5). Nine months later, MRI was performed in a 1.5T system. Fast spin-echo sequences (proton density-weighted and T2-weighted [T2W] images) were obtained (in-plane resolution: 350 × 350 microns, slice thickness: 3 mm). Magnetic resonance images were correlated with matched histopathological sections (n = 108). RESULTS: A significant correlation (p < 0.001) was observed for mean wall thickness and vessel wall area between MRI and histopathology (r = 0.87 and r = 0.85, respectively). The correlation was also present on subanalysis of the thoracic and upper part of the abdominal aorta, susceptible to respiratory motion artifacts. There was a significant correlation for plaque composition (p < 0.05) between MRI and histopathology for the analysis of lipidic (low signal on T2W, r = 0.81) and fibrous (high signal on T2W, r = 0.86) areas with Oil Red O staining. T2-weighted images showed greater contrast than proton density-weighted between these different components of the plaques as assessed by signal intensity ratio analysis with the mean difference in signal ratios of 0.47 (S.E. 0.012, adjusted for clustering of observations within lesions) being significantly different from 0 (t1 = 39.1, p = 0.016). CONCLUSIONS: In vivo noninvasive high resolution MRI accurately quantifies fibrotic and lipidic components of atherosclerosis in this model. This may permit the serial analysis of therapeutic strategies on atherosclerotic plaque stabilization.

Original languageEnglish (US)
Pages (from-to)1149-1154
Number of pages6
JournalJournal of the American College of Cardiology
Volume37
Issue number4
DOIs
StatePublished - Mar 15 2001

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Magnetic Resonance Imaging
Rabbits
Atherosclerotic Plaques
Protons
Thorax
Abdominal Aorta
Artifacts
Cluster Analysis
Atherosclerosis
Magnetic Resonance Spectroscopy
Staining and Labeling
Therapeutics

ASJC Scopus subject areas

  • Nursing(all)

Cite this

Atherosclerotic aortic component quantification by noninvasive magnetic resonance imaging : An in vivo study in rabbits. / Helft, Gérard; Worthley, Stephen G.; Fuster, Valentin; Zaman, Azfar G.; Schechter, Clyde B.; Osende, Julio I.; Rodriguez, Oswaldo J.; Fayad, Zahi A.; Fallon, John T.; Badimon, Juan J.

In: Journal of the American College of Cardiology, Vol. 37, No. 4, 15.03.2001, p. 1149-1154.

Research output: Contribution to journalArticle

Helft, G, Worthley, SG, Fuster, V, Zaman, AG, Schechter, CB, Osende, JI, Rodriguez, OJ, Fayad, ZA, Fallon, JT & Badimon, JJ 2001, 'Atherosclerotic aortic component quantification by noninvasive magnetic resonance imaging: An in vivo study in rabbits', Journal of the American College of Cardiology, vol. 37, no. 4, pp. 1149-1154. https://doi.org/10.1016/S0735-1097(01)01141-X
Helft, Gérard ; Worthley, Stephen G. ; Fuster, Valentin ; Zaman, Azfar G. ; Schechter, Clyde B. ; Osende, Julio I. ; Rodriguez, Oswaldo J. ; Fayad, Zahi A. ; Fallon, John T. ; Badimon, Juan J. / Atherosclerotic aortic component quantification by noninvasive magnetic resonance imaging : An in vivo study in rabbits. In: Journal of the American College of Cardiology. 2001 ; Vol. 37, No. 4. pp. 1149-1154.
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abstract = "OBJECTIVES: We sought to demonstrate the ability that noninvasive in vivo magnetic resonance imaging (MRI) has to quantify the different components within atherosclerotic plaque. BACKGROUND: Atherosclerotic plaque composition plays a critical role in both lesion stability and subsequent thrombogenicity. Noninvasive MRI is a promising tool for the characterization of plaque composition. METHODS: Thoracic and abdominal aortic atherosclerotic lesions were induced in rabbits (n = 5). Nine months later, MRI was performed in a 1.5T system. Fast spin-echo sequences (proton density-weighted and T2-weighted [T2W] images) were obtained (in-plane resolution: 350 × 350 microns, slice thickness: 3 mm). Magnetic resonance images were correlated with matched histopathological sections (n = 108). RESULTS: A significant correlation (p < 0.001) was observed for mean wall thickness and vessel wall area between MRI and histopathology (r = 0.87 and r = 0.85, respectively). The correlation was also present on subanalysis of the thoracic and upper part of the abdominal aorta, susceptible to respiratory motion artifacts. There was a significant correlation for plaque composition (p < 0.05) between MRI and histopathology for the analysis of lipidic (low signal on T2W, r = 0.81) and fibrous (high signal on T2W, r = 0.86) areas with Oil Red O staining. T2-weighted images showed greater contrast than proton density-weighted between these different components of the plaques as assessed by signal intensity ratio analysis with the mean difference in signal ratios of 0.47 (S.E. 0.012, adjusted for clustering of observations within lesions) being significantly different from 0 (t1 = 39.1, p = 0.016). CONCLUSIONS: In vivo noninvasive high resolution MRI accurately quantifies fibrotic and lipidic components of atherosclerosis in this model. This may permit the serial analysis of therapeutic strategies on atherosclerotic plaque stabilization.",
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T1 - Atherosclerotic aortic component quantification by noninvasive magnetic resonance imaging

T2 - An in vivo study in rabbits

AU - Helft, Gérard

AU - Worthley, Stephen G.

AU - Fuster, Valentin

AU - Zaman, Azfar G.

AU - Schechter, Clyde B.

AU - Osende, Julio I.

AU - Rodriguez, Oswaldo J.

AU - Fayad, Zahi A.

AU - Fallon, John T.

AU - Badimon, Juan J.

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Y1 - 2001/3/15

N2 - OBJECTIVES: We sought to demonstrate the ability that noninvasive in vivo magnetic resonance imaging (MRI) has to quantify the different components within atherosclerotic plaque. BACKGROUND: Atherosclerotic plaque composition plays a critical role in both lesion stability and subsequent thrombogenicity. Noninvasive MRI is a promising tool for the characterization of plaque composition. METHODS: Thoracic and abdominal aortic atherosclerotic lesions were induced in rabbits (n = 5). Nine months later, MRI was performed in a 1.5T system. Fast spin-echo sequences (proton density-weighted and T2-weighted [T2W] images) were obtained (in-plane resolution: 350 × 350 microns, slice thickness: 3 mm). Magnetic resonance images were correlated with matched histopathological sections (n = 108). RESULTS: A significant correlation (p < 0.001) was observed for mean wall thickness and vessel wall area between MRI and histopathology (r = 0.87 and r = 0.85, respectively). The correlation was also present on subanalysis of the thoracic and upper part of the abdominal aorta, susceptible to respiratory motion artifacts. There was a significant correlation for plaque composition (p < 0.05) between MRI and histopathology for the analysis of lipidic (low signal on T2W, r = 0.81) and fibrous (high signal on T2W, r = 0.86) areas with Oil Red O staining. T2-weighted images showed greater contrast than proton density-weighted between these different components of the plaques as assessed by signal intensity ratio analysis with the mean difference in signal ratios of 0.47 (S.E. 0.012, adjusted for clustering of observations within lesions) being significantly different from 0 (t1 = 39.1, p = 0.016). CONCLUSIONS: In vivo noninvasive high resolution MRI accurately quantifies fibrotic and lipidic components of atherosclerosis in this model. This may permit the serial analysis of therapeutic strategies on atherosclerotic plaque stabilization.

AB - OBJECTIVES: We sought to demonstrate the ability that noninvasive in vivo magnetic resonance imaging (MRI) has to quantify the different components within atherosclerotic plaque. BACKGROUND: Atherosclerotic plaque composition plays a critical role in both lesion stability and subsequent thrombogenicity. Noninvasive MRI is a promising tool for the characterization of plaque composition. METHODS: Thoracic and abdominal aortic atherosclerotic lesions were induced in rabbits (n = 5). Nine months later, MRI was performed in a 1.5T system. Fast spin-echo sequences (proton density-weighted and T2-weighted [T2W] images) were obtained (in-plane resolution: 350 × 350 microns, slice thickness: 3 mm). Magnetic resonance images were correlated with matched histopathological sections (n = 108). RESULTS: A significant correlation (p < 0.001) was observed for mean wall thickness and vessel wall area between MRI and histopathology (r = 0.87 and r = 0.85, respectively). The correlation was also present on subanalysis of the thoracic and upper part of the abdominal aorta, susceptible to respiratory motion artifacts. There was a significant correlation for plaque composition (p < 0.05) between MRI and histopathology for the analysis of lipidic (low signal on T2W, r = 0.81) and fibrous (high signal on T2W, r = 0.86) areas with Oil Red O staining. T2-weighted images showed greater contrast than proton density-weighted between these different components of the plaques as assessed by signal intensity ratio analysis with the mean difference in signal ratios of 0.47 (S.E. 0.012, adjusted for clustering of observations within lesions) being significantly different from 0 (t1 = 39.1, p = 0.016). CONCLUSIONS: In vivo noninvasive high resolution MRI accurately quantifies fibrotic and lipidic components of atherosclerosis in this model. This may permit the serial analysis of therapeutic strategies on atherosclerotic plaque stabilization.

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