Asymmetric sorting of Ash1p in yeast results from inhibition of translation by localization elements in the mRNA

Pascal Chartrand, Xiu Hua Meng, Stefan Huttelmaier, Damiane Donato, Robert H. Singer

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

ASH1 mRNA localizes at the bud tip of late-anaphase yeast, resulting in accumulation of Ash1p in the daughter nucleus. We show that disruption of the secondary structure, but not the protein coding, of all four ASH1 localization elements resulted in RNA and protein delocalization. Localization of both was incrementally restored by replacement of each of the four elements. However, transposition of the elements to the 3′UTR reinstated the RNA, but not the protein, localization. Interestingly, the mutant ASH1 mRNA was translated more efficiently, suggesting that asymmetry of Ash1p resulted from translational inhibition by the localization elements. In support of this, Ash1p asymmetry could be rescued by slowing its translation.

Original languageEnglish (US)
Pages (from-to)1319-1330
Number of pages12
JournalMolecular Cell
Volume10
Issue number6
DOIs
StatePublished - Dec 1 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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