Astrocytes regulate GFAP mRNA levels by cyclic AMP and protein kinase C-dependent mechanisms.

Bridget Shafit-Zagardo, A. Kume-Iwaki, J. E. Goldman

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Glial fibrillary acidic protein (GFAP) mRNA and protein levels in rat astrocyte cultures and in the human astrocytoma line U-373MG were examined in order to determine the effects of agents that regulate cAMP-dependent kinase and protein kinase C. Treatment of cells with dibutyryl cAMP or forskolin and 3-isobutyl-1-methylxanthine increased steady-state GFAP mRNA levels. Short-term treatment of cells with phorbol-12-myristate-13-acetate (PMA) increased GFAP mRNA levels, but prolonged treatment of cells with PMA or 1-oleoyl-2-acetyl-rac-glycerol produced a dramatic decrease in GFAP mRNA; 4-beta-phorbol had no effect. Thus, both cAMP-dependent kinase and protein kinase C may exert regulatory roles in determining GFAP mRNA levels. Nuclear run-off studies showed no change in GFAP mRNA synthesis after cAMP or PMA treatment, suggesting post-transcriptional mechanisms. Western blot analysis revealed that the effect of PMA on U-373MG cells shows specificity in that GFA protein levels decline, while those of other major cytoskeletal proteins were unaltered.

Original languageEnglish (US)
Pages (from-to)346-354
Number of pages9
JournalGLIA
Volume1
Issue number5
StatePublished - 1988

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Adenylate Kinase
Glial Fibrillary Acidic Protein
Astrocytes
Cyclic AMP
Protein Kinase C
Messenger RNA
Acetates
Cyclic AMP-Dependent Protein Kinases
1-Methyl-3-isobutylxanthine
Cytoskeletal Proteins
Astrocytoma
Colforsin
Western Blotting
phorbol-12-myristate

ASJC Scopus subject areas

  • Immunology

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Astrocytes regulate GFAP mRNA levels by cyclic AMP and protein kinase C-dependent mechanisms. / Shafit-Zagardo, Bridget; Kume-Iwaki, A.; Goldman, J. E.

In: GLIA, Vol. 1, No. 5, 1988, p. 346-354.

Research output: Contribution to journalArticle

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abstract = "Glial fibrillary acidic protein (GFAP) mRNA and protein levels in rat astrocyte cultures and in the human astrocytoma line U-373MG were examined in order to determine the effects of agents that regulate cAMP-dependent kinase and protein kinase C. Treatment of cells with dibutyryl cAMP or forskolin and 3-isobutyl-1-methylxanthine increased steady-state GFAP mRNA levels. Short-term treatment of cells with phorbol-12-myristate-13-acetate (PMA) increased GFAP mRNA levels, but prolonged treatment of cells with PMA or 1-oleoyl-2-acetyl-rac-glycerol produced a dramatic decrease in GFAP mRNA; 4-beta-phorbol had no effect. Thus, both cAMP-dependent kinase and protein kinase C may exert regulatory roles in determining GFAP mRNA levels. Nuclear run-off studies showed no change in GFAP mRNA synthesis after cAMP or PMA treatment, suggesting post-transcriptional mechanisms. Western blot analysis revealed that the effect of PMA on U-373MG cells shows specificity in that GFA protein levels decline, while those of other major cytoskeletal proteins were unaltered.",
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