Astrocyte-shed extracellular vesicles regulate the peripheral leukocyte response to inflammatory brain lesions

Alex M. Dickens, Luis B. Tovar-Y-Romo, Seung Wan Yoo, Amanda L. Trout, Mihyun Bae, Marlene Kanmogne, Bezawit Megra, Dionna W. Williams, Kennith W. Witwer, Mar Gacias, Nino Tabatadze, Robert N. Cole, Patrizia Casaccia, Joan W. Berman, Daniel C. Anthony, Norman J. Haughey

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Brain injury induces a peripheral acute cytokine response that directs the transmigration of leukocytes into the brain. Because this brain-to-peripheral immune communication affects patient recovery, understanding its regulation is important. Using a mouse model of inflammatory brain injury, we set out to find a soluble mediator for this phenomenon. We found that extracellular vesicles (EVs) shed from astrocytes in response to intracerebral injection of interleukin-1β (IL-1β) rapidly entered into peripheral circulation and promoted the transmigration of leukocytes through modulation of the peripheral acute cytokine response. Bioinformatic analysis of the protein and microRNA cargo of EVs identified peroxisome proliferator-activated receptor α (PPARα) as a primary molecular target of astrocyte-shed EVs. We confirmed in mice that astrocytic EVs promoted the transmigration of leukocytes into the brain by inhibiting PPARα, resulting in the increase of nuclear factor κB (NF-κB) activity that triggered the production of cytokines in liver. These findings expand our understanding of the mechanisms regulating communication between the brain and peripheral immune system and identify astrocytic EVs as a molecular regulator of the immunological response to inflammatory brain damage.

Original languageEnglish (US)
Article numbereaai7696
JournalScience Signaling
Volume10
Issue number473
DOIs
StatePublished - Apr 4 2017

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Astrocytes
Brain
Leukocytes
Peroxisome Proliferator-Activated Receptors
Cytokines
Brain Injuries
Communication
Computational Biology
MicroRNAs
Interleukin-1
Immune System
Immune system
Bioinformatics
Extracellular Vesicles
Liver
Injections
Modulation
Recovery
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Dickens, A. M., Tovar-Y-Romo, L. B., Yoo, S. W., Trout, A. L., Bae, M., Kanmogne, M., ... Haughey, N. J. (2017). Astrocyte-shed extracellular vesicles regulate the peripheral leukocyte response to inflammatory brain lesions. Science Signaling, 10(473), [eaai7696]. https://doi.org/10.1126/scisignal.aai7696

Astrocyte-shed extracellular vesicles regulate the peripheral leukocyte response to inflammatory brain lesions. / Dickens, Alex M.; Tovar-Y-Romo, Luis B.; Yoo, Seung Wan; Trout, Amanda L.; Bae, Mihyun; Kanmogne, Marlene; Megra, Bezawit; Williams, Dionna W.; Witwer, Kennith W.; Gacias, Mar; Tabatadze, Nino; Cole, Robert N.; Casaccia, Patrizia; Berman, Joan W.; Anthony, Daniel C.; Haughey, Norman J.

In: Science Signaling, Vol. 10, No. 473, eaai7696, 04.04.2017.

Research output: Contribution to journalArticle

Dickens, AM, Tovar-Y-Romo, LB, Yoo, SW, Trout, AL, Bae, M, Kanmogne, M, Megra, B, Williams, DW, Witwer, KW, Gacias, M, Tabatadze, N, Cole, RN, Casaccia, P, Berman, JW, Anthony, DC & Haughey, NJ 2017, 'Astrocyte-shed extracellular vesicles regulate the peripheral leukocyte response to inflammatory brain lesions', Science Signaling, vol. 10, no. 473, eaai7696. https://doi.org/10.1126/scisignal.aai7696
Dickens, Alex M. ; Tovar-Y-Romo, Luis B. ; Yoo, Seung Wan ; Trout, Amanda L. ; Bae, Mihyun ; Kanmogne, Marlene ; Megra, Bezawit ; Williams, Dionna W. ; Witwer, Kennith W. ; Gacias, Mar ; Tabatadze, Nino ; Cole, Robert N. ; Casaccia, Patrizia ; Berman, Joan W. ; Anthony, Daniel C. ; Haughey, Norman J. / Astrocyte-shed extracellular vesicles regulate the peripheral leukocyte response to inflammatory brain lesions. In: Science Signaling. 2017 ; Vol. 10, No. 473.
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