Astrocyte-derived monocyte-chemoattractant protein-1 directs the transmigration of leukocytes across a model of the human blood-brain barrier

Jonathan M. Weiss, Sherry A. Downie, William D. Lyman, Joan W. Berman

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Abstract

The migration of leukocytes across the blood-brain barrier (BBB) into the central nervous system is critical in the pathogenesis of central nervous system inflammatory diseases. The production of chemokines, such as monocyte- chemoattractant protein-1 (MCP-1), by endothelial cells (EC) and astrocytes may initiate and amplify this process. Using a coculture of human EC and astrocytes to model the BBB, we demonstrated that exogenous MCP-1 induces the transmigration of monocytes in a dose-dependent manner. TNF-α, IFN-γ, or IL-1β treatment of cocultures also induced significant migration of monocytes that correlates with the induction of MCP-1 protein. TGF-β, previously shown to induce MCP-1 expression in astrocytes, but not in EC, caused migration of monocytes across cocultures, but not across EC grown alone. Monocytes and lymphocytes transmigrated across cytokine-treated cocultures in greater numbers than across EC alone. Astrocytes were the main source of cytokine-induced MCP-1, supporting a role for astrocytes in facilitating leukocyte transmigration. A blocking Ab to MCP-1 inhibited MCP- 1- and cytokine-induced transmigration of monocytes by 85-90%. Cytokine treatment of cocultures also resulted in the transmigration of activated, CD69-positive lymphocytes. The MCP-1-mediated transmigration of monocytes across cocultures was blocked using an Ab to ICAM-1 and inhibited by 55% using an Ab to E-selectin. These data suggest a central role for astrocyte- derived MCP-1 in directing the migration of monocytes and lymphocytes across the BBB.

Original languageEnglish (US)
Pages (from-to)6896-6903
Number of pages8
JournalJournal of Immunology
Volume161
Issue number12
StatePublished - Dec 15 1998

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Chemokine CCL2
Blood-Brain Barrier
Astrocytes
Leukocytes
Coculture Techniques
Monocytes
Endothelial Cells
Cytokines
Lymphocytes
E-Selectin
Central Nervous System Diseases
Intercellular Adhesion Molecule-1
Interleukin-1
Chemokines
Cell Movement
Central Nervous System

ASJC Scopus subject areas

  • Immunology

Cite this

Astrocyte-derived monocyte-chemoattractant protein-1 directs the transmigration of leukocytes across a model of the human blood-brain barrier. / Weiss, Jonathan M.; Downie, Sherry A.; Lyman, William D.; Berman, Joan W.

In: Journal of Immunology, Vol. 161, No. 12, 15.12.1998, p. 6896-6903.

Research output: Contribution to journalArticle

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