Association of Human Antibodies to Arabinomannan with Enhanced Mycobacterial Opsonophagocytosis and Intracellular Growth Reduction

Tingting Chen, Caroline Blanc, Anke Z. Eder, Rafael Prados-Rosales, Ana Camila Oliveira Souza, Ryung S. Kim, Aharona Glatman-Freedman, Maju Joe, Yu Bai, Todd L. Lowary, Rachel Tanner, Michael J. Brennan, Helen A. Fletcher, Helen McShane, Arturo Casadevall, Jacqueline M. Achkar

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background. The relevance of antibodies (Abs) in the defense against Mycobacterium tuberculosis infection remains uncertain. We investigated the role of Abs to the mycobacterial capsular polysaccharide arabinomannan (AM) and its oligosaccharide (OS) fragments in humans. Methods. Sera obtained from 29 healthy adults before and after primary or secondary bacillus Calmette-Guerin (BCG) vaccination were assessed for Ab responses to AM via enzyme-linked immunosorbent assays, and to AM OS epitopes via novel glycan microarrays. Effects of prevaccination and postvaccination sera on BCG phagocytosis and intracellular survival were assessed in human macrophages. Results. Immunoglobulin G (IgG) responses to AM increased significantly 4-8 weeks after vaccination (P

Original languageEnglish (US)
Pages (from-to)300-310
Number of pages11
JournalJournal of Infectious Diseases
Volume214
Issue number2
DOIs
StatePublished - Jul 15 2016

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Antibodies
Mycobacterium bovis
Growth
Oligosaccharides
Polysaccharides
Vaccination
Mycobacterium Infections
Serum
Mycobacterium tuberculosis
Phagocytosis
Epitopes
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Macrophages
Survival
arabinomannan

Keywords

  • Antibody-mediated immunity
  • Bacterial capsule
  • Human antibodies
  • Immune response
  • Immunoglobulin
  • Mycobacterium bovis BCG
  • Mycobacterium tuberculosis
  • Oligosaccharide
  • Polysaccharide
  • Tuberculosis

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Association of Human Antibodies to Arabinomannan with Enhanced Mycobacterial Opsonophagocytosis and Intracellular Growth Reduction. / Chen, Tingting; Blanc, Caroline; Eder, Anke Z.; Prados-Rosales, Rafael; Souza, Ana Camila Oliveira; Kim, Ryung S.; Glatman-Freedman, Aharona; Joe, Maju; Bai, Yu; Lowary, Todd L.; Tanner, Rachel; Brennan, Michael J.; Fletcher, Helen A.; McShane, Helen; Casadevall, Arturo; Achkar, Jacqueline M.

In: Journal of Infectious Diseases, Vol. 214, No. 2, 15.07.2016, p. 300-310.

Research output: Contribution to journalArticle

Chen, T, Blanc, C, Eder, AZ, Prados-Rosales, R, Souza, ACO, Kim, RS, Glatman-Freedman, A, Joe, M, Bai, Y, Lowary, TL, Tanner, R, Brennan, MJ, Fletcher, HA, McShane, H, Casadevall, A & Achkar, JM 2016, 'Association of Human Antibodies to Arabinomannan with Enhanced Mycobacterial Opsonophagocytosis and Intracellular Growth Reduction', Journal of Infectious Diseases, vol. 214, no. 2, pp. 300-310. https://doi.org/10.1093/infdis/jiw141
Chen, Tingting ; Blanc, Caroline ; Eder, Anke Z. ; Prados-Rosales, Rafael ; Souza, Ana Camila Oliveira ; Kim, Ryung S. ; Glatman-Freedman, Aharona ; Joe, Maju ; Bai, Yu ; Lowary, Todd L. ; Tanner, Rachel ; Brennan, Michael J. ; Fletcher, Helen A. ; McShane, Helen ; Casadevall, Arturo ; Achkar, Jacqueline M. / Association of Human Antibodies to Arabinomannan with Enhanced Mycobacterial Opsonophagocytosis and Intracellular Growth Reduction. In: Journal of Infectious Diseases. 2016 ; Vol. 214, No. 2. pp. 300-310.
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