Association of herpesvirus with fibropapillomatosis of the green turtle Chelonia mydas and the loggerhead turtle Caretta caretta in Florida

Joel K. Lackovich, Daniel R. Brown, Bruce L. Homer, Richard L. Garber, Douglas R. Mader, Ritchie H. Moretti, Amy D. Patterson, Lawrence H. Herbst, Jorge Oros, Elliott R. Jacobson, Sadie S. Curry, Paul A. Klein

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Sea turtle fibropapillomatosis (FP) is a disease marked by proliferation of benign but debilitating cutaneous fibropapillomas and occasional visceral fibromas. Transmission experiments have implicated a chloroform-sensitive transforming agent present in filtered cell-free tumor homogenates in the etiology of FP. In this study, consensus primer PCR methodology was used to test the association of a chelonian herpesvirus with fibropapillomatosis. Fibropapilloma and skin samples were obtained from 17 green and 2 loggerhead turtles affected with FP stranded along the Florida coastline. Ninety-three cutaneous and visceral tumors from the 19 turtles, and 33 skin samples from 16 of the turtles, were tested. All turtles affected with FP had herpesvirus associated with their tumors as detected by PCR. Ninety-six percent (89/93) of the tumors, but only 9% (3/33) of the skin samples, from affected turtles contained detectable herpesvirus. The skin samples that contained herpesvirus were all within 2 cm of a fibropapilloma. Also, 1 of 11 scar tissue samples from sites where fibropapillomas had been removed 2 to 51 wk earlier from 5 green turtles contained detectable herpesvirus. None of 18 normal skin samples from 2 green and 2 loggerhead turtles stranded without FP contained herpesvirus. The data indicated that herpesvirus was detectable only within or close to tumors. To determine if the same virus infected both turtle species, partial nucleotide sequences of the herpesvirus DNA polymerase gene were determined from 6 loggerhead and 2 green turtle samples. The sequences predicted that herpesvirus of loggerhead turtles differed from those of green turtles by only 1 of 60 amino acids in the sequence examined, indicating that a chelonian herpesvirus exhibiting minor intratypic variation was the only herpesvirus present in tumors of both green and loggerhead turtles. The FP-associated herpesvirus resisted cultivation on chelonian cell lines which support the replication of other chelonian herpesviruses. These results lead to the conclusion that a chelonian herpesvirus is regularly associated with fibropapillomatosis and is not merely an incidental finding in affected turtles.

Original languageEnglish (US)
Pages (from-to)89-97
Number of pages9
JournalDiseases of Aquatic Organisms
Volume37
Issue number2
StatePublished - Jul 30 1999

Fingerprint

Chelonia mydas
Caretta caretta
turtle
turtles
skin (animal)
neoplasms
tumor
sampling
skin
fibroma
sea turtles
DNA-directed DNA polymerase
chloroform
etiology
amino acid sequences
cell lines
nucleotide sequences
coasts
viruses

Keywords

  • Caretta caretta
  • Chelonia mydas
  • Chelonian herpesvirus
  • Fibropapillomatosis
  • Sea turtles

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

Lackovich, J. K., Brown, D. R., Homer, B. L., Garber, R. L., Mader, D. R., Moretti, R. H., ... Klein, P. A. (1999). Association of herpesvirus with fibropapillomatosis of the green turtle Chelonia mydas and the loggerhead turtle Caretta caretta in Florida. Diseases of Aquatic Organisms, 37(2), 89-97.

Association of herpesvirus with fibropapillomatosis of the green turtle Chelonia mydas and the loggerhead turtle Caretta caretta in Florida. / Lackovich, Joel K.; Brown, Daniel R.; Homer, Bruce L.; Garber, Richard L.; Mader, Douglas R.; Moretti, Ritchie H.; Patterson, Amy D.; Herbst, Lawrence H.; Oros, Jorge; Jacobson, Elliott R.; Curry, Sadie S.; Klein, Paul A.

In: Diseases of Aquatic Organisms, Vol. 37, No. 2, 30.07.1999, p. 89-97.

Research output: Contribution to journalArticle

Lackovich, JK, Brown, DR, Homer, BL, Garber, RL, Mader, DR, Moretti, RH, Patterson, AD, Herbst, LH, Oros, J, Jacobson, ER, Curry, SS & Klein, PA 1999, 'Association of herpesvirus with fibropapillomatosis of the green turtle Chelonia mydas and the loggerhead turtle Caretta caretta in Florida', Diseases of Aquatic Organisms, vol. 37, no. 2, pp. 89-97.
Lackovich, Joel K. ; Brown, Daniel R. ; Homer, Bruce L. ; Garber, Richard L. ; Mader, Douglas R. ; Moretti, Ritchie H. ; Patterson, Amy D. ; Herbst, Lawrence H. ; Oros, Jorge ; Jacobson, Elliott R. ; Curry, Sadie S. ; Klein, Paul A. / Association of herpesvirus with fibropapillomatosis of the green turtle Chelonia mydas and the loggerhead turtle Caretta caretta in Florida. In: Diseases of Aquatic Organisms. 1999 ; Vol. 37, No. 2. pp. 89-97.
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