Association of endothelial proliferation with the magnitude of weight loss during calorie restriction

Katarzyna Korybalska, Ewelina Swora-Cwynar, Joanna Łuczak, Alina Kanikowska, Natasza Czepulis, Rafał Rutkowski, Andrzej Bręborowicz, Marian Grzymisławski, Janusz Witowski

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objectives: Substantial weight loss through intense dietary regimens is thought to ameliorate endothelial dysfunction in obesity. It is less clear whether similar improvements can be achieved with modest dietary interventions. This study aimed to identify the parameters of endothelial cell status in obesity that are affected by mild calorie restriction. Methods: Human umbilical vein endothelial cells (EA.hy926 line) in culture were exposed pairwise to serum from 57 individuals with simple obesity (BMI > 30 kg/m2) collected before and after 8-week dietary intervention with energy deficit of 300–500 kcal/day. Results: Analysis of endothelial transcriptome suggested that the intervention could impact on endothelial cell growth. Cell proliferation was measured with the MTT test and verified by [3H]-thymidine incorporation. The participants were categorized according to a change in proliferation over time. Significant decrease in endothelial cell proliferation correlated with the extent of weight loss in men, but not in women. This effect corresponded with changes in serum levels of leptin and adiponectin, but was not related to serum concentrations of several known angiogenic mediators (VEGF, MCP-1, TSP-1, MMP-9, angiopoietin-2). Conclusion: Direction and magnitude of changes in serum-induced endothelial cell proliferation identifies patients with the greatest weight loss in response to modest calorie restriction.

Original languageEnglish (US)
Pages (from-to)407-419
Number of pages13
JournalAngiogenesis
Volume19
Issue number3
DOIs
StatePublished - Jul 1 2016
Externally publishedYes

Fingerprint

Endothelial cells
Weight Loss
Endothelial Cells
Cell proliferation
Obesity
Cell Proliferation
Serum
Angiopoietin-2
Thrombospondin 1
Adiponectin
Human Umbilical Vein Endothelial Cells
Gene Expression Profiling
Leptin
Matrix Metalloproteinases
Thymidine
Vascular Endothelial Growth Factor A
Cell growth
Cell culture
Growth

Keywords

  • Cell proliferation
  • Endothelium
  • Obesity
  • Thrifty phenotype
  • Weight loss

ASJC Scopus subject areas

  • Cancer Research
  • Physiology
  • Clinical Biochemistry

Cite this

Korybalska, K., Swora-Cwynar, E., Łuczak, J., Kanikowska, A., Czepulis, N., Rutkowski, R., ... Witowski, J. (2016). Association of endothelial proliferation with the magnitude of weight loss during calorie restriction. Angiogenesis, 19(3), 407-419. https://doi.org/10.1007/s10456-016-9516-7

Association of endothelial proliferation with the magnitude of weight loss during calorie restriction. / Korybalska, Katarzyna; Swora-Cwynar, Ewelina; Łuczak, Joanna; Kanikowska, Alina; Czepulis, Natasza; Rutkowski, Rafał; Bręborowicz, Andrzej; Grzymisławski, Marian; Witowski, Janusz.

In: Angiogenesis, Vol. 19, No. 3, 01.07.2016, p. 407-419.

Research output: Contribution to journalArticle

Korybalska, K, Swora-Cwynar, E, Łuczak, J, Kanikowska, A, Czepulis, N, Rutkowski, R, Bręborowicz, A, Grzymisławski, M & Witowski, J 2016, 'Association of endothelial proliferation with the magnitude of weight loss during calorie restriction', Angiogenesis, vol. 19, no. 3, pp. 407-419. https://doi.org/10.1007/s10456-016-9516-7
Korybalska K, Swora-Cwynar E, Łuczak J, Kanikowska A, Czepulis N, Rutkowski R et al. Association of endothelial proliferation with the magnitude of weight loss during calorie restriction. Angiogenesis. 2016 Jul 1;19(3):407-419. https://doi.org/10.1007/s10456-016-9516-7
Korybalska, Katarzyna ; Swora-Cwynar, Ewelina ; Łuczak, Joanna ; Kanikowska, Alina ; Czepulis, Natasza ; Rutkowski, Rafał ; Bręborowicz, Andrzej ; Grzymisławski, Marian ; Witowski, Janusz. / Association of endothelial proliferation with the magnitude of weight loss during calorie restriction. In: Angiogenesis. 2016 ; Vol. 19, No. 3. pp. 407-419.
@article{83d46d2299e04390875c40ac94caf569,
title = "Association of endothelial proliferation with the magnitude of weight loss during calorie restriction",
abstract = "Objectives: Substantial weight loss through intense dietary regimens is thought to ameliorate endothelial dysfunction in obesity. It is less clear whether similar improvements can be achieved with modest dietary interventions. This study aimed to identify the parameters of endothelial cell status in obesity that are affected by mild calorie restriction. Methods: Human umbilical vein endothelial cells (EA.hy926 line) in culture were exposed pairwise to serum from 57 individuals with simple obesity (BMI > 30 kg/m2) collected before and after 8-week dietary intervention with energy deficit of 300–500 kcal/day. Results: Analysis of endothelial transcriptome suggested that the intervention could impact on endothelial cell growth. Cell proliferation was measured with the MTT test and verified by [3H]-thymidine incorporation. The participants were categorized according to a change in proliferation over time. Significant decrease in endothelial cell proliferation correlated with the extent of weight loss in men, but not in women. This effect corresponded with changes in serum levels of leptin and adiponectin, but was not related to serum concentrations of several known angiogenic mediators (VEGF, MCP-1, TSP-1, MMP-9, angiopoietin-2). Conclusion: Direction and magnitude of changes in serum-induced endothelial cell proliferation identifies patients with the greatest weight loss in response to modest calorie restriction.",
keywords = "Cell proliferation, Endothelium, Obesity, Thrifty phenotype, Weight loss",
author = "Katarzyna Korybalska and Ewelina Swora-Cwynar and Joanna Łuczak and Alina Kanikowska and Natasza Czepulis and Rafał Rutkowski and Andrzej Bręborowicz and Marian Grzymisławski and Janusz Witowski",
year = "2016",
month = "7",
day = "1",
doi = "10.1007/s10456-016-9516-7",
language = "English (US)",
volume = "19",
pages = "407--419",
journal = "Angiogenesis",
issn = "0969-6970",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Association of endothelial proliferation with the magnitude of weight loss during calorie restriction

AU - Korybalska, Katarzyna

AU - Swora-Cwynar, Ewelina

AU - Łuczak, Joanna

AU - Kanikowska, Alina

AU - Czepulis, Natasza

AU - Rutkowski, Rafał

AU - Bręborowicz, Andrzej

AU - Grzymisławski, Marian

AU - Witowski, Janusz

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Objectives: Substantial weight loss through intense dietary regimens is thought to ameliorate endothelial dysfunction in obesity. It is less clear whether similar improvements can be achieved with modest dietary interventions. This study aimed to identify the parameters of endothelial cell status in obesity that are affected by mild calorie restriction. Methods: Human umbilical vein endothelial cells (EA.hy926 line) in culture were exposed pairwise to serum from 57 individuals with simple obesity (BMI > 30 kg/m2) collected before and after 8-week dietary intervention with energy deficit of 300–500 kcal/day. Results: Analysis of endothelial transcriptome suggested that the intervention could impact on endothelial cell growth. Cell proliferation was measured with the MTT test and verified by [3H]-thymidine incorporation. The participants were categorized according to a change in proliferation over time. Significant decrease in endothelial cell proliferation correlated with the extent of weight loss in men, but not in women. This effect corresponded with changes in serum levels of leptin and adiponectin, but was not related to serum concentrations of several known angiogenic mediators (VEGF, MCP-1, TSP-1, MMP-9, angiopoietin-2). Conclusion: Direction and magnitude of changes in serum-induced endothelial cell proliferation identifies patients with the greatest weight loss in response to modest calorie restriction.

AB - Objectives: Substantial weight loss through intense dietary regimens is thought to ameliorate endothelial dysfunction in obesity. It is less clear whether similar improvements can be achieved with modest dietary interventions. This study aimed to identify the parameters of endothelial cell status in obesity that are affected by mild calorie restriction. Methods: Human umbilical vein endothelial cells (EA.hy926 line) in culture were exposed pairwise to serum from 57 individuals with simple obesity (BMI > 30 kg/m2) collected before and after 8-week dietary intervention with energy deficit of 300–500 kcal/day. Results: Analysis of endothelial transcriptome suggested that the intervention could impact on endothelial cell growth. Cell proliferation was measured with the MTT test and verified by [3H]-thymidine incorporation. The participants were categorized according to a change in proliferation over time. Significant decrease in endothelial cell proliferation correlated with the extent of weight loss in men, but not in women. This effect corresponded with changes in serum levels of leptin and adiponectin, but was not related to serum concentrations of several known angiogenic mediators (VEGF, MCP-1, TSP-1, MMP-9, angiopoietin-2). Conclusion: Direction and magnitude of changes in serum-induced endothelial cell proliferation identifies patients with the greatest weight loss in response to modest calorie restriction.

KW - Cell proliferation

KW - Endothelium

KW - Obesity

KW - Thrifty phenotype

KW - Weight loss

UR - http://www.scopus.com/inward/record.url?scp=84973165140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973165140&partnerID=8YFLogxK

U2 - 10.1007/s10456-016-9516-7

DO - 10.1007/s10456-016-9516-7

M3 - Article

AN - SCOPUS:84973165140

VL - 19

SP - 407

EP - 419

JO - Angiogenesis

JF - Angiogenesis

SN - 0969-6970

IS - 3

ER -