Association of chronic hepatitis c infection with T-cell phenotypes in HIV-negative and HIV-positive women

Mark H. Kuniholm, Xianhong Xie, Kathryn Anastos, Robert C. Kaplan, Xiaonan (Nan) Xue, Andrea Kovacs, Marion G. Peters, Eric C. Seaberg, Audrey L. French, Mary A. Young, Michael Augenbraun, Jeffrey A. Martinson, Kristin A. Bush, Alan L. Landay, Howard Strickler

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Hepatitis C virus (HCV) viremia is thought to have broad systemic effects on the cellular immune system that go beyond its impact on just those T cells that are HCV specific. However, previous studies of chronic HCV and circulating T-cell subsets (activation and differentiation phenotypes) in HIV negatives used general population controls, rather than a risk-appropriate comparison group. Studies in HIV positives did not address overall immune status (total CD4<sup>+</sup> count). Methods: We used fresh blood from HIV-positive and at-risk HIV-negative women, with and without chronic HCV, to measure percentages of activated CD4<sup>+</sup> and CD8<sup>+</sup> T cells, Tregs, and T-cell differentiation phenotypes (naive, central memory, effector memory (EM), and terminally differentiated effector). This included 158 HIV negatives and 464 HIV positives, of whom 18 and 63, respectively, were HCV viremic. Results: In multivariate models of HIV negatives, HCV viremia was associated with 25% fewer naive CD4<sup>+</sup> (P = 0.03), 33% more EM CD4<sup>+</sup> (P = 0.0002), and 37% fewer central memory CD8<sup>+</sup> (P = 0.02) T cells. Among HIV positives, we observed only 1 of these 3 relationships: higher percentage of EM CD4<sup>+</sup> among HCV viremic women. Furthermore, the association with EM CD4<sup>+</sup> among HIV positives was limited to individuals with diminished immune status (total CD4<sup>+</sup> count ≤500 cells/μL), as were associations of HCV viremia with higher percentages of activated CD4<sup>+</sup> and Tregs. Among HIV positives with high CD4<sup>+</sup> count, no significant associations were observed. Conclusions: These data suggest that HCV viremia in HIV negatives is associated with accelerated T-cell differentiation, but among HIV positives, the impact of HCV viremia is less straightforward and varies by total CD4<sup>+</sup> count.

Original languageEnglish (US)
Pages (from-to)295-303
Number of pages9
JournalJournal of Acquired Immune Deficiency Syndromes
Volume67
Issue number3
StatePublished - 2014

Fingerprint

Chronic Hepatitis
Hepacivirus
HIV
T-Lymphocytes
Phenotype
Infection
Viremia
CD4 Lymphocyte Count
Chronic Hepatitis C
Cell Differentiation
Population Control
T-Lymphocyte Subsets
Immune System

Keywords

  • Activation
  • Differentiation
  • Hepatitis C virus
  • HIV
  • Phenotype
  • T cell

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)
  • Medicine(all)

Cite this

Association of chronic hepatitis c infection with T-cell phenotypes in HIV-negative and HIV-positive women. / Kuniholm, Mark H.; Xie, Xianhong; Anastos, Kathryn; Kaplan, Robert C.; Xue, Xiaonan (Nan); Kovacs, Andrea; Peters, Marion G.; Seaberg, Eric C.; French, Audrey L.; Young, Mary A.; Augenbraun, Michael; Martinson, Jeffrey A.; Bush, Kristin A.; Landay, Alan L.; Strickler, Howard.

In: Journal of Acquired Immune Deficiency Syndromes, Vol. 67, No. 3, 2014, p. 295-303.

Research output: Contribution to journalArticle

Kuniholm, MH, Xie, X, Anastos, K, Kaplan, RC, Xue, XN, Kovacs, A, Peters, MG, Seaberg, EC, French, AL, Young, MA, Augenbraun, M, Martinson, JA, Bush, KA, Landay, AL & Strickler, H 2014, 'Association of chronic hepatitis c infection with T-cell phenotypes in HIV-negative and HIV-positive women', Journal of Acquired Immune Deficiency Syndromes, vol. 67, no. 3, pp. 295-303.
Kuniholm, Mark H. ; Xie, Xianhong ; Anastos, Kathryn ; Kaplan, Robert C. ; Xue, Xiaonan (Nan) ; Kovacs, Andrea ; Peters, Marion G. ; Seaberg, Eric C. ; French, Audrey L. ; Young, Mary A. ; Augenbraun, Michael ; Martinson, Jeffrey A. ; Bush, Kristin A. ; Landay, Alan L. ; Strickler, Howard. / Association of chronic hepatitis c infection with T-cell phenotypes in HIV-negative and HIV-positive women. In: Journal of Acquired Immune Deficiency Syndromes. 2014 ; Vol. 67, No. 3. pp. 295-303.
@article{166f0f3df85a471dbec8d2eebf38a52d,
title = "Association of chronic hepatitis c infection with T-cell phenotypes in HIV-negative and HIV-positive women",
abstract = "Background: Hepatitis C virus (HCV) viremia is thought to have broad systemic effects on the cellular immune system that go beyond its impact on just those T cells that are HCV specific. However, previous studies of chronic HCV and circulating T-cell subsets (activation and differentiation phenotypes) in HIV negatives used general population controls, rather than a risk-appropriate comparison group. Studies in HIV positives did not address overall immune status (total CD4+ count). Methods: We used fresh blood from HIV-positive and at-risk HIV-negative women, with and without chronic HCV, to measure percentages of activated CD4+ and CD8+ T cells, Tregs, and T-cell differentiation phenotypes (naive, central memory, effector memory (EM), and terminally differentiated effector). This included 158 HIV negatives and 464 HIV positives, of whom 18 and 63, respectively, were HCV viremic. Results: In multivariate models of HIV negatives, HCV viremia was associated with 25{\%} fewer naive CD4+ (P = 0.03), 33{\%} more EM CD4+ (P = 0.0002), and 37{\%} fewer central memory CD8+ (P = 0.02) T cells. Among HIV positives, we observed only 1 of these 3 relationships: higher percentage of EM CD4+ among HCV viremic women. Furthermore, the association with EM CD4+ among HIV positives was limited to individuals with diminished immune status (total CD4+ count ≤500 cells/μL), as were associations of HCV viremia with higher percentages of activated CD4+ and Tregs. Among HIV positives with high CD4+ count, no significant associations were observed. Conclusions: These data suggest that HCV viremia in HIV negatives is associated with accelerated T-cell differentiation, but among HIV positives, the impact of HCV viremia is less straightforward and varies by total CD4+ count.",
keywords = "Activation, Differentiation, Hepatitis C virus, HIV, Phenotype, T cell",
author = "Kuniholm, {Mark H.} and Xianhong Xie and Kathryn Anastos and Kaplan, {Robert C.} and Xue, {Xiaonan (Nan)} and Andrea Kovacs and Peters, {Marion G.} and Seaberg, {Eric C.} and French, {Audrey L.} and Young, {Mary A.} and Michael Augenbraun and Martinson, {Jeffrey A.} and Bush, {Kristin A.} and Landay, {Alan L.} and Howard Strickler",
year = "2014",
language = "English (US)",
volume = "67",
pages = "295--303",
journal = "Journal of Acquired Immune Deficiency Syndromes",
issn = "1525-4135",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Association of chronic hepatitis c infection with T-cell phenotypes in HIV-negative and HIV-positive women

AU - Kuniholm, Mark H.

AU - Xie, Xianhong

AU - Anastos, Kathryn

AU - Kaplan, Robert C.

AU - Xue, Xiaonan (Nan)

AU - Kovacs, Andrea

AU - Peters, Marion G.

AU - Seaberg, Eric C.

AU - French, Audrey L.

AU - Young, Mary A.

AU - Augenbraun, Michael

AU - Martinson, Jeffrey A.

AU - Bush, Kristin A.

AU - Landay, Alan L.

AU - Strickler, Howard

PY - 2014

Y1 - 2014

N2 - Background: Hepatitis C virus (HCV) viremia is thought to have broad systemic effects on the cellular immune system that go beyond its impact on just those T cells that are HCV specific. However, previous studies of chronic HCV and circulating T-cell subsets (activation and differentiation phenotypes) in HIV negatives used general population controls, rather than a risk-appropriate comparison group. Studies in HIV positives did not address overall immune status (total CD4+ count). Methods: We used fresh blood from HIV-positive and at-risk HIV-negative women, with and without chronic HCV, to measure percentages of activated CD4+ and CD8+ T cells, Tregs, and T-cell differentiation phenotypes (naive, central memory, effector memory (EM), and terminally differentiated effector). This included 158 HIV negatives and 464 HIV positives, of whom 18 and 63, respectively, were HCV viremic. Results: In multivariate models of HIV negatives, HCV viremia was associated with 25% fewer naive CD4+ (P = 0.03), 33% more EM CD4+ (P = 0.0002), and 37% fewer central memory CD8+ (P = 0.02) T cells. Among HIV positives, we observed only 1 of these 3 relationships: higher percentage of EM CD4+ among HCV viremic women. Furthermore, the association with EM CD4+ among HIV positives was limited to individuals with diminished immune status (total CD4+ count ≤500 cells/μL), as were associations of HCV viremia with higher percentages of activated CD4+ and Tregs. Among HIV positives with high CD4+ count, no significant associations were observed. Conclusions: These data suggest that HCV viremia in HIV negatives is associated with accelerated T-cell differentiation, but among HIV positives, the impact of HCV viremia is less straightforward and varies by total CD4+ count.

AB - Background: Hepatitis C virus (HCV) viremia is thought to have broad systemic effects on the cellular immune system that go beyond its impact on just those T cells that are HCV specific. However, previous studies of chronic HCV and circulating T-cell subsets (activation and differentiation phenotypes) in HIV negatives used general population controls, rather than a risk-appropriate comparison group. Studies in HIV positives did not address overall immune status (total CD4+ count). Methods: We used fresh blood from HIV-positive and at-risk HIV-negative women, with and without chronic HCV, to measure percentages of activated CD4+ and CD8+ T cells, Tregs, and T-cell differentiation phenotypes (naive, central memory, effector memory (EM), and terminally differentiated effector). This included 158 HIV negatives and 464 HIV positives, of whom 18 and 63, respectively, were HCV viremic. Results: In multivariate models of HIV negatives, HCV viremia was associated with 25% fewer naive CD4+ (P = 0.03), 33% more EM CD4+ (P = 0.0002), and 37% fewer central memory CD8+ (P = 0.02) T cells. Among HIV positives, we observed only 1 of these 3 relationships: higher percentage of EM CD4+ among HCV viremic women. Furthermore, the association with EM CD4+ among HIV positives was limited to individuals with diminished immune status (total CD4+ count ≤500 cells/μL), as were associations of HCV viremia with higher percentages of activated CD4+ and Tregs. Among HIV positives with high CD4+ count, no significant associations were observed. Conclusions: These data suggest that HCV viremia in HIV negatives is associated with accelerated T-cell differentiation, but among HIV positives, the impact of HCV viremia is less straightforward and varies by total CD4+ count.

KW - Activation

KW - Differentiation

KW - Hepatitis C virus

KW - HIV

KW - Phenotype

KW - T cell

UR - http://www.scopus.com/inward/record.url?scp=84922012319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922012319&partnerID=8YFLogxK

M3 - Article

C2 - 25314250

AN - SCOPUS:84922012319

VL - 67

SP - 295

EP - 303

JO - Journal of Acquired Immune Deficiency Syndromes

JF - Journal of Acquired Immune Deficiency Syndromes

SN - 1525-4135

IS - 3

ER -