Abstract
A highly purified preparation of the eucaryotic initiation factor eIF-2 from calf liver which forms a ternary complex with GTP and Met-tRNAfMet also exhibits a potent GDP binding activity. The factor preparation specifically forms a binary complex with GDP, other ribonucleoside diphosphates and GTP are inactive. Evidence is presented indicating that the GTP-dependent Met-tRNAfMet binding and binary complex formation with GDP are mediated by the same protein which has an apparent molecular weight of 67,000 as judged by glycerol density gradient centrifugation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 586-592 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 76 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 23 1977 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Stringer, E. A., Chaudhuri, A., & Maitra, U. (1977). Association of a GDP binding activity with initiation factor eIF-2 from calf liver. Biochemical and Biophysical Research Communications, 76(2), 586-592. https://doi.org/10.1016/0006-291X(77)90764-1