Association of a GDP binding activity with initiation factor eIF-2 from calf liver

Evan A. Stringer; Asok Chaudhuri; Umadas Maitra

doi:10.1016/0006-291X(77)90764-1

Association of a GDP binding activity with initiation factor eIF-2 from calf liver

Evan A. Stringer, Asok Chaudhuri, Umadas Maitra

Developmental & Molecular Biology

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8 Scopus citations

Abstract

A highly purified preparation of the eucaryotic initiation factor eIF-2 from calf liver which forms a ternary complex with GTP and Met-tRNA_f^Met also exhibits a potent GDP binding activity. The factor preparation specifically forms a binary complex with GDP, other ribonucleoside diphosphates and GTP are inactive. Evidence is presented indicating that the GTP-dependent Met-tRNA_f^Met binding and binary complex formation with GDP are mediated by the same protein which has an apparent molecular weight of 67,000 as judged by glycerol density gradient centrifugation.

Original language	English (US)
Pages (from-to)	586-592
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	76
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(77)90764-1
State	Published - May 23 1977

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Association of a GDP binding activity with initiation factor eIF-2 from calf liver",

abstract = "A highly purified preparation of the eucaryotic initiation factor eIF-2 from calf liver which forms a ternary complex with GTP and Met-tRNAfMet also exhibits a potent GDP binding activity. The factor preparation specifically forms a binary complex with GDP, other ribonucleoside diphosphates and GTP are inactive. Evidence is presented indicating that the GTP-dependent Met-tRNAfMet binding and binary complex formation with GDP are mediated by the same protein which has an apparent molecular weight of 67,000 as judged by glycerol density gradient centrifugation.",

author = "Stringer, {Evan A.} and Asok Chaudhuri and Umadas Maitra",

note = "Funding Information: ACKNOWLEDGEMENTS: This work was supported by grants stitutes of Health and the American Cancer Society. Research Awardee of the American Cancer Society.",

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AU - Stringer, Evan A.

AU - Chaudhuri, Asok

AU - Maitra, Umadas

N1 - Funding Information: ACKNOWLEDGEMENTS: This work was supported by grants stitutes of Health and the American Cancer Society. Research Awardee of the American Cancer Society.

PY - 1977/5/23

Y1 - 1977/5/23

N2 - A highly purified preparation of the eucaryotic initiation factor eIF-2 from calf liver which forms a ternary complex with GTP and Met-tRNAfMet also exhibits a potent GDP binding activity. The factor preparation specifically forms a binary complex with GDP, other ribonucleoside diphosphates and GTP are inactive. Evidence is presented indicating that the GTP-dependent Met-tRNAfMet binding and binary complex formation with GDP are mediated by the same protein which has an apparent molecular weight of 67,000 as judged by glycerol density gradient centrifugation.

AB - A highly purified preparation of the eucaryotic initiation factor eIF-2 from calf liver which forms a ternary complex with GTP and Met-tRNAfMet also exhibits a potent GDP binding activity. The factor preparation specifically forms a binary complex with GDP, other ribonucleoside diphosphates and GTP are inactive. Evidence is presented indicating that the GTP-dependent Met-tRNAfMet binding and binary complex formation with GDP are mediated by the same protein which has an apparent molecular weight of 67,000 as judged by glycerol density gradient centrifugation.

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Association of a GDP binding activity with initiation factor eIF-2 from calf liver

Abstract

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