Association of a GDP binding activity with initiation factor eIF-2 from calf liver

Evan A. Stringer; Asok Chaudhuri; Umadas Maitra

doi:10.1016/0006-291X(77)90764-1

Association of a GDP binding activity with initiation factor eIF-2 from calf liver

Evan A. Stringer, Asok Chaudhuri, Umadas Maitra

Developmental & Molecular Biology

Research output: Contribution to journal › Article

8 Scopus citations

Abstract

A highly purified preparation of the eucaryotic initiation factor eIF-2 from calf liver which forms a ternary complex with GTP and Met-tRNA_f^Met also exhibits a potent GDP binding activity. The factor preparation specifically forms a binary complex with GDP, other ribonucleoside diphosphates and GTP are inactive. Evidence is presented indicating that the GTP-dependent Met-tRNA_f^Met binding and binary complex formation with GDP are mediated by the same protein which has an apparent molecular weight of 67,000 as judged by glycerol density gradient centrifugation.

Original language	English (US)
Pages (from-to)	586-592
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	76
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(77)90764-1
Publication status	Published - May 23 1977

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ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Cite this

Stringer, E. A., Chaudhuri, A., & Maitra, U. (1977). Association of a GDP binding activity with initiation factor eIF-2 from calf liver. Biochemical and Biophysical Research Communications, 76(2), 586-592. https://doi.org/10.1016/0006-291X(77)90764-1

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10.1016/0006-291X(77)90764-1