Assignment of human sprouty 4 gene to chromosome segment 5q32-33 and analysis of its pattern of expression

Hua Liu, Jin Zhong Chen, Shao Hua Gu, Jian Liang Dai, En Pang Zhao, Lu Huang, Wang Xiang Xu, Yi Xie, Yu Min Mao

Research output: Contribution to journalArticle

Abstract

The human sprouty 4 (SPYR4) gene was localized to chromosome band 5q32-33 by screening the Stanford radiation hybrid G3 panel using a SPRY4-specific primer pair for PCR. Northern blot analysis revealed two different mRNAs (5 kb and 2 kb) in liver, skeletal muscle, heart, lung, kidney, spleen, placenta and small intestine. Reverse transcriptase-PCR analysis showed that SPYR4 was expressed in all tested tissues to different levels.

Original languageEnglish (US)
Pages (from-to)23-26
Number of pages4
JournalJournal of Genetics
Volume82
Issue number1-2
StatePublished - Apr 2003
Externally publishedYes

Fingerprint

Radiation Hybrid Mapping
Reverse Transcriptase Polymerase Chain Reaction
Northern Blotting
Placenta
Small Intestine
Skeletal Muscle
Spleen
Chromosomes
Kidney
Polymerase Chain Reaction
Lung
Messenger RNA
Liver
Genes

Keywords

  • Expression pattern
  • Radioactive hybridization (RH)
  • SPRY4

ASJC Scopus subject areas

  • Genetics

Cite this

Liu, H., Chen, J. Z., Gu, S. H., Dai, J. L., Zhao, E. P., Huang, L., ... Mao, Y. M. (2003). Assignment of human sprouty 4 gene to chromosome segment 5q32-33 and analysis of its pattern of expression. Journal of Genetics, 82(1-2), 23-26.

Assignment of human sprouty 4 gene to chromosome segment 5q32-33 and analysis of its pattern of expression. / Liu, Hua; Chen, Jin Zhong; Gu, Shao Hua; Dai, Jian Liang; Zhao, En Pang; Huang, Lu; Xu, Wang Xiang; Xie, Yi; Mao, Yu Min.

In: Journal of Genetics, Vol. 82, No. 1-2, 04.2003, p. 23-26.

Research output: Contribution to journalArticle

Liu, H, Chen, JZ, Gu, SH, Dai, JL, Zhao, EP, Huang, L, Xu, WX, Xie, Y & Mao, YM 2003, 'Assignment of human sprouty 4 gene to chromosome segment 5q32-33 and analysis of its pattern of expression', Journal of Genetics, vol. 82, no. 1-2, pp. 23-26.
Liu, Hua ; Chen, Jin Zhong ; Gu, Shao Hua ; Dai, Jian Liang ; Zhao, En Pang ; Huang, Lu ; Xu, Wang Xiang ; Xie, Yi ; Mao, Yu Min. / Assignment of human sprouty 4 gene to chromosome segment 5q32-33 and analysis of its pattern of expression. In: Journal of Genetics. 2003 ; Vol. 82, No. 1-2. pp. 23-26.
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